BtaEX6018674 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107285194-107287026:-
Coord C1 exon
chr3:107286508-107287026
Coord A exon
chr3:107285573-107285749
Coord C2 exon
chr3:107285194-107285317
Length
177 bp
Sequences
Splice sites
3' ss Seq
CCCTTCTTTCTCTGGCACAGTGT
3' ss Score
8.67
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
TGTCTCAGCAGCAGAATTTCATCCTGGCTACGCAGTCAGCCCAGGCCTTCCTGGACCAGCACGGCCACAGCCTCACACCCGAGGAGCAACAGCTGCTGCAAGAGAAGCTCGGAGAGCTGAAGGAGCAGTATGCGACTTCCCTGGCCCAGTCGGAGGCAGAACTGAAGCAGGTGCAAACACTGCGGGACGAGCTGCAGAAGTTTCTGCAGGACCATCGGGAGTTTGAGAACTGGCTGGAGCGATCTGAGAAAGAGCTGGAGAGCATGCATCAGGGAGGCAGCAGCCCCGAGGCCCTTCCCGCCCTGCTGAAGCGGCAGGGGAGCTTCTCGGAGGATGTCATTTCCCACAAAGGAGACCTGCGGTTTGTGACGATCTCAGGACAGAAGGTCTTGGACACAGGAAACAGCCTCGATGATGGCAGGGAAGCGTCAGCAACCGGAACCTTAGTGAAGGCCAAGCTGAAAGACGCAACAGAAAGATACAGCACTCTCCATGCAGAG
Seq A exon
TGTACACGCCTGGGCACTCACCTGAACATGCTGCTAGGCCAGTATCAGCAGTTTCAGAGCAGCGCTGACAGCCTGCAGGCCTGGATGAAAGCTTGCGAGGCCAGTGTGAAGAAGCTCCTCTCAGACACGGTTGCCTCTGACCCGGGGGTCCTGCAACAGCAGTTTGCAACCACAAAG
Seq C2 exon
CAGTTGCAGGAGGAATTGGCTGAGCACCAAGTACCTGTAGAAAAACTCCAAAAAGTAGCTCATGACCTCATGGAAATTGAAGGGGAGCCAGCCCCCGACCGCAAGCATGTTCAAGAAACTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'54-55,'54-54,55-55=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.341 A=0.000 C2=0.595
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PU(92.0=60.1)
A:
PF0043516=Spectrin=PD(6.2=11.9),PF0043516=Spectrin=PU(41.4=78.0)
C2:
PF0043516=Spectrin=FE(36.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGGAGACCTGCGGTTTGTG
R:
GCTGGCTCCCCTTCAATTTCC
Band lengths:
245-422
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]