Special

MmuEX0027459 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123132237-123133992:-
Coord C1 exon
chr4:123133474-123133992
Coord A exon
chr4:123132601-123132777
Coord C2 exon
chr4:123132237-123132360
Length
177 bp
Sequences
Splice sites
3' ss Seq
TTTTTATTTCTTTGGCTTAGTGT
3' ss Score
8.12
5' ss Seq
AAGGTAGGC
5' ss Score
10.08
Exon sequences
Seq C1 exon
TGTCTCAGCAGCAGAATTTCATTGTGGCCACCCAGTCAGTTCAGTCCTTCCTGGACCAACACAGTCACAATCTCACACCTGAGGAGCGACAGAAGCTTCAGGAGAAGCTGGGTGAGCTTAAGGAGCAATATGCAGCCTCCCTTGCCCGGTCAGAGGCAGAGCTGAAGCAGACGCAGGCTCTACGAGATGAACTGCAGAAGTTCCTGCAAGACCACAAGGAATTTGAAAACTGGCTACAACAATCTGAGAATGAGCTGGATAGTATGCATAAGGGAGGCAGCAGTCCTGAAGCTCTGAACTCCTTGCTAAAGCGGCAAGGAAGCTTCTCTGAGGATGTCATTTCCCACAAAGGAGACTTGAGATTTGTGACTATCTCAGGGCAGAAAGTCTTAGAAACAGAAAACAATTTTGAGGAAGGCCAAGAGCCATCAGCTACCAGAAACTTAGTGAATGAAAAACTGAAGGATGCAACAGAAAGATACACCACTCTCCACTCTAAG
Seq A exon
TGTATACGATTGGGCTCTCATCTGAGCATGTTGCTAGGCCAGTATCAGCAATTCCAGAGCAGCGCTGACAGCCTGCAGGCCTGGGTGTTGACTTGTGAGGCCAGCGTGGGGAAGCTTCTCTCAGACACAGTTGCCTCTGACCCCGGAGTCCTGCAACAGCAGCTTGCAACAACAAAG
Seq C2 exon
CAGTTACAGGAAGAATTGGCTGAACACCAAGTACCTGTAGAAAAGCTTCAAAAAGCAGCTCATGACCTACTGGACATTGAAGGAGAGCCAGCCCTGGACTGTAGGCCTATTCAAGAAACTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'63-62,'63-61,64-62
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.410 A=0.016 C2=0.229
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0043516=Spectrin=PU(96.3=60.1)

							
A:
PF0043516=Spectrin=PD(1.9=3.4),PF0043516=Spectrin=PU(41.4=78.0)

							
C2:
PF0043516=Spectrin=FE(36.9=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000081324f2416A





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000080755, ENSMUSP00000081324, ENSMUSP00000081324f6186A, ENSMUSP00000095507, ENSMUSP00000095508, ENSMUSP00000095509, ENSMUSP00000101819, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000101831f2417A, ENSMUSP00000114568, ENSMUSP00000119885
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39358697-39358873 
HIGH PSI
HsaEX6030184
(MACF1)
Human
(hg19)
chr1:39824369-39824545 
HIGH PSI
HsaEX6030184
(MACF1)
Rat
(rn6)
chr5:141140324-141140500 
ALTERNATIVE
RnoEX0051748
(Macf1)
Cow
(bosTau6)
chr3:107285573-107285749 
HIGH PSI
BtaEX6018674
(MACF1)
Chicken
(galGal4)
chrUn_AADN03026614:282-458 
Chicken
(galGal3)
chr23:5666562-5666738 
HIGH PSI
GgaEX6025265
(MACF1)
Zebrafish
(danRer10)
chr19:35548190-35548366 
HIGH PSI
DreEX6073710
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AACTCCTTGCTAAAGCGGCAA

				
R: 
ACAGGTACTTGGTGTTCAGCC

				
Band lengths: 
242-419

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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