DreEX6079217 @ danRer10
Exon Skipping
Gene
ENSDARG00000058543 | lama5
Description
laminin, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-030131-9823]
Coordinates
chr23:9639635-9644288:-
Coord C1 exon
chr23:9644142-9644288
Coord A exon
chr23:9639852-9639999
Coord C2 exon
chr23:9639635-9639765
Length
148 bp
Sequences
Splice sites
3' ss Seq
TTGACTTGTCTGTCTGTCAGGCT
3' ss Score
7.25
5' ss Seq
TCGGTCAGC
5' ss Score
4.32
Exon sequences
Seq C1 exon
GTGTTTGTGAAAGTGGAGGGTGACCGGGGGTCTCTGACTGTGGACGGCATCGACACACAGAACAAGAGAGTGTCTGCTGGAGGGAAGAGTATGTTCGCTGCTCCGCTCTATATCGGAGGACTTCCTGTAGACCACAGCGCAGCCATG
Seq A exon
GCTGGATTCGTGGGCTGTGTTCGGGACCTGAAACTGAACGAGGTGTCTCTTCAGAGCCCATCTGTCAGTGTAGGTGTGACGCCCTGTTACCAGCAGCCTTTGCAGCCCGGCGCTCACTTCTCCAGTCGTGGAGGATTCATGACCATCG
Seq C2 exon
ACGAGTCGCTGGTTTTAGGACAGGACCTGGAGATCCAATTGGAGGTCCGTCTGGATTCAGGCTCAGGGCTTCTGCTGCACACTGGAGCCAAGAAAACACACCAGCTGAGTGTGTATCTGGAACAAGGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058543-'85-82,'85-81,86-82=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.102 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0221019=Laminin_G_2=FE(39.7=100)
A:
PF0221019=Laminin_G_2=PD(11.6=28.0)
C2:
PF0221019=Laminin_G_2=PU(22.0=63.6)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTTTGTGAAAGTGGAGGGTG
R:
TTTCTTGGCTCCAGTGTGCAG
Band lengths:
242-390
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]