MmuEX0026071 @ mm10
Exon Skipping
Gene
ENSMUSG00000015647 | Lama5
Description
laminin, alpha 5 [Source:MGI Symbol;Acc:MGI:105382]
Coordinates
chr2:180176373-180177899:-
Coord C1 exon
chr2:180177735-180177899
Coord A exon
chr2:180177491-180177644
Coord C2 exon
chr2:180176373-180176764
Length
154 bp
Sequences
Splice sites
3' ss Seq
TATCCATCTGCTCCTTGCAGGTG
3' ss Score
10.64
5' ss Seq
TAGGTATGT
5' ss Score
7.76
Exon sequences
Seq C1 exon
GTGTCCGTCCGCTGGGGAATGCAGCAGATCCAGCTTGTGGTGGACGGCAGCCAGACCTGGAGCCAGAAGGCTCTCCACCATCGGGTCCCCAGGGCAGAGCGACCACAGCCCTACACCCTCTCTGTAGGAGGTCTTCCTGCCAGCAGTTACAGTTCCAAGCTCCCT
Seq A exon
GTGTCTGTGGGGTTCAGCGGCTGTCTGAAGAAATTACAGCTGGATAAGCGGCCACTGAGGACCCCAACGCAAATGGTGGGGGTCACACCCTGTGTCTCAGGCCCCCTGGAAGATGGCCTGTTCTTCCCAGGCAGTGAGGGAGTTGTCACATTAG
Seq C2 exon
AGTCTTCAACTGCTCGGCCAGAGCTCCCTGCCTACCGAGGATGCTTGAGGAAGCTGCTGATCAATGGGGCCCCTGTCAACGTGACTGCTTCTGTACAAATCCAGGGGGCAGTGGGGATGCGCGGATGCCCCTCAGGAACCCTAGCACTTTCCAAGCAGGGAAAGGCACTGACCCAGAGGCAGGCCAAGCCCAGTGTCTCCCCGCTACTTTGGCATTGAGGGTTCCCAGACCTTGGGGTTTGCCTACACTTTCTATGAATAACAAGTCATTTCTGGTTTACACTGTCTTTTTAGAGGAAAAGGACTCTGTAGAACAGATATTATATTTATACTTTGTTCCTCCTGCCCAACAACTGTCAGTACTGAGATCTGAAATTAAAATGACTTGTTTGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015647_MULTIEX1-6/9=5-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.255 A=0.038 C2=0.181
Domain overlap (PFAM):
C1:
PF0221019=Laminin_G_2=FE(41.2=100)
A:
PF0221019=Laminin_G_2=PD(12.2=30.8)
C2:
PF0221019=Laminin_G_2=PD(18.5=31.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTCTTCCTGCCAGCAGTTAC
R:
ACCCTCAATGCCAAAGTAGCG
Band lengths:
258-412
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types