DreEX6081726 @ danRer10
Exon Skipping
Gene
ENSDARG00000079291 | rapgef3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:ZFIN;Acc:ZDB-GENE-090312-38]
Coordinates
chr23:33505207-33510148:+
Coord C1 exon
chr23:33505207-33505332
Coord A exon
chr23:33507783-33507964
Coord C2 exon
chr23:33510058-33510148
Length
182 bp
Sequences
Splice sites
3' ss Seq
TTGTATTTGCTGTACTGCAGGTG
3' ss Score
9.39
5' ss Seq
TGTGTGAGT
5' ss Score
5.84
Exon sequences
Seq C1 exon
ACACCTGATAAAGAGCAGCTGGGGCCGGAGAAGAGCACCATGGACACTCTGGAGCAGATTTGCTCCAAGGACATGGCCAGTCAACACACCAGCTACGACTGGGAACTCTTCATGGCCATGCATGAG
Seq A exon
GTGGAGCTTGTCTACTATGTTTTCGGACGGGAAAAGTTCCCTGGATCCACCACAGCAAACCTCGAGCGGTTTGTTCGTCGCTTCAATGAGGTTCAGTACTGGGTTGTGACTGAACTGTGTCTCTGTGAAGACCTGGGCAAGAGAGCCATACTGCTGAAGAAGTTCATCAAGATGGCTGTTGT
Seq C2 exon
TTTGAAAGAGCAGAAGAACCTCAACTCTTTTTTCGCAGTAATGTTTGGCCTCAGCAACAGTGCAGTGCAGAGGCTCAATAAAACATGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079291-'23-24,'23-23,24-24=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.190 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=PU(12.9=57.1)
A:
PF0061714=RasGEF=FE(32.3=100)
C2:
PF0061714=RasGEF=FE(16.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGATAAAGAGCAGCTGGGGC
R:
CCCATGTTTTATTGAGCCTCTGC
Band lengths:
211-393
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]