DreEX6083613 @ danRer10
Exon Skipping
Gene
ENSDARG00000073792 | man1b1a
Description
mannosidase, alpha, class 1B, member 1a [Source:ZFIN;Acc:ZDB-GENE-091116-34]
Coordinates
chr21:14135744-14140604:-
Coord C1 exon
chr21:14140468-14140604
Coord A exon
chr21:14138734-14138843
Coord C2 exon
chr21:14135744-14135935
Length
110 bp
Sequences
Splice sites
3' ss Seq
TGATGTTTCTGGACTTCTAGCTG
3' ss Score
5.35
5' ss Seq
CAGGTAATG
5' ss Score
9.43
Exon sequences
Seq C1 exon
AGAAAACCTCCTAGCAAGCGTGGTCCCCCAGCGCTACAGAAGCGAATGAACGTGTCCGACTGGCAGCTGAAAGATGGAGAAGAAGGCTTGATCAAGACAGATGAAGACGGAGCAAAAGAAGCAAAAACAGTCATTAG
Seq A exon
CTGGCGCGGGGCGATGATTGAGGCAGAGCAGGGCACTGAGCTTCAGTCCACCATTCAGGGAAAGGATGCAACTGTGGTTGATGCTGAGGAAGCTGCTGACTTAAAAACAG
Seq C2 exon
GGACATTGAAGCCAGTGGATCGTGTGGAAGCGGTGCAGGAGGCCTTCAGGCATGCATGGAAAGGTTATAAGGCTTTTGCATGGGGTCATGATGAGCTGAAGCCACTATCTAAGACTCATGGAGAGTGGTTTGGTCTGGGCCTCACGCTCATAGATGCACTGGACACCATGTGGATCCTTGGTCTGAAAGACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000073792-'6-6,'6-5,9-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.674 A=0.553 C2=0.015
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0153215=Glyco_hydro_47=PU(11.4=76.9)
Main Skipping Isoform:
ENSDART00000114715fB3700
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGCGAATGAACGTGTCCGA
R:
GTGCATCTATGAGCGTGAGGC
Band lengths:
258-368
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]