DreEX6085014 @ danRer10
Exon Skipping
Gene
ENSDARG00000013755 | actn3a
Description
actinin alpha 3a [Source:ZFIN;Acc:ZDB-GENE-000329-9]
Coordinates
chr21:27363355-27363985:+
Coord C1 exon
chr21:27363355-27363440
Coord A exon
chr21:27363552-27363644
Coord C2 exon
chr21:27363755-27363985
Length
93 bp
Sequences
Splice sites
3' ss Seq
TCAGTCACACTGTCCTCCAGGCT
3' ss Score
6.02
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
Exon sequences
Seq C1 exon
ACATTGTGAACACTCCAAAACCTGATGAGAAGGCCATCATGACCTATGTGTCATGTTTCTACCATGCTTTTGCTGGTGCAGAGCAG
Seq A exon
GCTGAGACAGCAGCTAACAGGATCTGCAAAGTCCTGGCAGTGAACCAGGAAAATGAGAAGCTGATGGAAGAATACGAGAAACTGGCCAGCGAG
Seq C2 exon
CTTCTGGAGTGGATTCAAAGGACCATTCCCTGGTTAGAGAACCGTGTGGCTGAGCAGACCATGCATGCCATGCAGCAGAAGCTAGAGGATTTTAGGGATTATCGTCGTGTGCATAAGCCACCCAAGGTTCAAGAGAAGTGTCAGCTGGAGATCAACTTCAACACCCTACAGACCAAGCTAAGGCTCAGCAACAGGCCTGCCTTCATGCCTTCCGAGGGCAAAATGGTTTCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000013755-'12-12,'12-11,13-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.026
Domain overlap (PFAM):
C1:
PF0030726=CH=PD(23.1=82.8)
A:
PF0043516=Spectrin=PU(9.9=35.5)
C2:
PF0043516=Spectrin=FE(68.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCCATCATGACCTATGTGTCA
R:
GTGGCTTATGCACACGACGAT
Band lengths:
176-269
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]