DreEX6095164 @ danRer10
Exon Skipping
Gene
ENSDARG00000014527 | pdk3a
Description
pyruvate dehydrogenase kinase, isozyme 3a [Source:ZFIN;Acc:ZDB-GENE-061220-3]
Coordinates
chr24:24805217-24809395:-
Coord C1 exon
chr24:24809254-24809395
Coord A exon
chr24:24809100-24809171
Coord C2 exon
chr24:24805217-24805401
Length
72 bp
Sequences
Splice sites
3' ss Seq
GCTACCAAATCTCTCCACAGGTA
3' ss Score
8.77
5' ss Seq
TGAGTAAGC
5' ss Score
5.78
Exon sequences
Seq C1 exon
GTCGGGACAATGCATGTGAGAAGACGTCCTACATGTTCCTGCGGAAGGAGCTGCCGGTGCGTCTTGCTAACACTATGAGAGAGGTCAATCTACTGCCGGACAGACTCCTCAGTCAACCTTCAGTCAAACTAGTGCAGAAATG
Seq A exon
GTACTTGCAAAGCTTTTTAGAGCTTCTAGAGTTTGAGAACAGGAAGCCAGAAGATGGACATGCCCTGAATGA
Seq C2 exon
CTTCTTAGAGACTCTTATTGAGATCAGGAACAGACACAATGATGTGGTCCCAACTATGGCCCAGGGTGTGATAGAATATAAGGAGAAGTTTGGATTCGATCCATTCGTCAGCTCCAACATCCAGTACTTTCTGGACCGCTTCTACACCAACCGAATTTCCTTTCGCATGCTCATCAACCAGCACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014527-'3-6,'3-2,4-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF104364=BCDHK_Adom3=PU(25.2=97.3)
A:
PF104364=BCDHK_Adom3=FE(16.8=100)
C2:
PF104364=BCDHK_Adom3=FE(43.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACGTCCTACATGTTCCTGCG
R:
GGCCATAGTTGGGACCACATC
Band lengths:
181-253
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]