DreEX6099400 @ danRer10
Exon Skipping
Gene
ENSDARG00000052748 | capn1b
Description
calpain 1, (mu/I) large subunit b [Source:ZFIN;Acc:ZDB-GENE-060503-491]
Coordinates
chr22:26289155-26291937:-
Coord C1 exon
chr22:26291863-26291937
Coord A exon
chr22:26291284-26291444
Coord C2 exon
chr22:26289155-26289324
Length
161 bp
Sequences
Splice sites
3' ss Seq
ATGACTGAATTTGTTCTCAGGAT
3' ss Score
7.17
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
Exon sequences
Seq C1 exon
TTCGTCCGAGTGGAATGGGATTGATCCATCGGAAAGAGAGGAACTGAACAACCACATGGAGGATGGCGAGTTTTG
Seq A exon
GATGTCATTTCAGGAGTTCAAACGTCAGTTTTCTCGTCTGGAGATCTGCAACCTCACTCCTGATGCTCTGAGTGATGATTCCCAACATTTCTGGAACACAATTCAGTTTAACGGCACTTGGAGGAAAGGAAGTACAGCAGGAGGCTGCAGGAACAATCCCA
Seq C2 exon
ACACATTCTGGATAAACCCTCAATATAAGATCACACTACTGGAGGAAGATGATGATCCTGAGGACAATGAGGTGGCCTGCACCTTCATGGTGGCTTTGATGCAGAAGGACAGACGGCGTTTCCGCAAGCAGGGACAGGACATGCACACCATCGGCTTCGCCTTATATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052748-'9-10,'9-8,10-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.462 A=0.036 C2=0.035
Domain overlap (PFAM):
C1:
PF0064816=Peptidase_C2=FE(8.3=100)
A:
PF0064816=Peptidase_C2=PD(6.3=34.5),PF0106717=Calpain_III=PU(15.8=43.6)
C2:
PF0106717=Calpain_III=FE(36.8=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGAGTGGAATGGGATTGATCC
R:
GAAGCCGATGGTGTGCATGTC
Band lengths:
228-389
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]