DreEX6102110 @ danRer10
Exon Skipping
Gene
ENSDARG00000023886 | cacna2d4b
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4b [Source:ZFIN;Acc:ZDB-GENE-100422-18]
Coordinates
chr25:18963115-18967175:-
Coord C1 exon
chr25:18967097-18967175
Coord A exon
chr25:18965194-18965327
Coord C2 exon
chr25:18963115-18963273
Length
134 bp
Sequences
Splice sites
3' ss Seq
CTTATATTAATTATTTACAGGTT
3' ss Score
7.89
5' ss Seq
GTGGTGAGT
5' ss Score
8.95
Exon sequences
Seq C1 exon
GTACGAGTTTTCACTTACCTCATTGGCAGAGACATGACCTTCTCAGAAAATGTCAAATGGATTGCCTGCAACAATAAAG
Seq A exon
GTTTTTATGCTCATGTCTCCACCCTGGCTGACGTACAGGAGAACGTCATGGAGTATCTTCACGTTTTGAGTCGGCCGATGGTCATCAATCACGACCATGACATAATCTGGACTGAGGCATATATGGACAGTGTG
Seq C2 exon
GTGTTCAAGAGCAAGGCTCACAGTCTGCTGTTCATGACGTCGGTGGCCATGCCAGTGTTCAGTAAGAAGAAAGAAACACTTTCTCATGGCATTTTGCTAGGTGTTGTGGGAACTGACATCCCCCTGCTGGAGGTCATGAAACTTGCCCCGCGATATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000023886-'15-16,'15-13,16-16=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF137681=VWA_3=FE(15.7=100)
A:
PF137681=VWA_3=PD(2.4=8.9),PF0274313=Cache_1=PU(8.5=17.8)
C2:
PF0274313=Cache_1=FE(55.3=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGAGTTTTCACTTACCTCATTGGC
R:
TTATATCGCGGGGCAAGTTTCA
Band lengths:
234-368
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]