DreEX6103800 @ danRer10
Exon Skipping
Gene
ENSDARG00000062174 | cacnb4b
Description
calcium channel, voltage-dependent, beta 4b subunit [Source:ZFIN;Acc:ZDB-GENE-060421-7907]
Coordinates
chr6:13975571-13992078:-
Coord C1 exon
chr6:13991951-13992078
Coord A exon
chr6:13980526-13980545
Coord C2 exon
chr6:13975571-13975651
Length
20 bp
Sequences
Splice sites
3' ss Seq
TTTTCCTTCTCTTTGTACAGGCA
3' ss Score
10.4
5' ss Seq
GTGGTAAGT
5' ss Score
10.36
Exon sequences
Seq C1 exon
AAGTTTAATAATGACTGGTGGATTGGGAGGCTGGTAAAAGAAGGCTGTGAGATTGGCTTTATCCCCAGTCCCCTGAAGCTGGAGAATATCAGAGCACAGCTGGAACAGAAGAAAAGCAGAGTACAAGG
Seq A exon
GCAAGCAGAAACAGAAAGTG
Seq C2 exon
ACTGAACATGTGGCTCCCTATGATGTTGTGCCTTCCATGAGGCCTGTGGTTCTGGTTGGTCCATCCCTGAAAGGATATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062174-'8-18,'8-15,12-18=AN
Average complexity
A_C2
Mappability confidence:
92%=86=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.159 A=0.670 C2=0.018
Domain overlap (PFAM):
C1:
PF0001823=SH3_1=PD(52.4=51.2)
A:
NO
C2:
PF0062516=Guanylate_kin=PU(8.3=55.6)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTATCCCCAGTCCCCTGAAGC
R:
GGCACAACATCATAGGGAGCC
Band lengths:
102-122
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]