DreINT0025513 @ danRer10
Intron Retention
Gene
ENSDARG00000014674 | acsl3b
Description
acyl-CoA synthetase long-chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492]
Coordinates
chr2:47643764-47644094:+
Coord C1 exon
chr2:47643764-47643873
Coord A exon
chr2:47643874-47643955
Coord C2 exon
chr2:47643956-47644094
Length
82 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
3' ss Seq
CATGATCCTCCATCTCTCAGGCT
3' ss Score
8.33
Exon sequences
Seq C1 exon
TGGTGACTCTTTACTCTACACTGGGAGGATCAGCTATAGCTCATGGTCTGAACGAGACTGAGGTCACACACATCGTCACAAGCAAAGACCTGCTGCAGAGCCGCCTCAAG
Seq A exon
GTAAAAACAAAACCCACACAATAACACAATCAACCAGAAAGCACTTTCTCATTCCTGCTCTGCATGATCCTCCATCTCTCAG
Seq C2 exon
GCTATTCTGTTAGAAGTCCCCAGACTCAAGCACATTATAGTGGTCGACACGAAACCCACAAGCTGGCCTGATCTCCCCAGAGGCATCATGGTACATAACATGGCAGCGGTGCAAGAATTGGGATCCAAACCTGAAAACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014674:ENSDART00000141974:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.5=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(9.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGAGACTGAGGTCACACACA
R:
ACCGCTGCCATGTTATGTACC
Band lengths:
169-251
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]