DreINT0026249 @ danRer10
Intron Retention
Gene
ENSDARG00000076270 | adamts13
Description
ADAM metallopeptidase with thrombospondin type 1 motif, 13 [Source:ZFIN;Acc:ZDB-GENE-070814-7]
Coordinates
chr8:45264431-45264911:-
Coord C1 exon
chr8:45264787-45264911
Coord A exon
chr8:45264578-45264786
Coord C2 exon
chr8:45264431-45264577
Length
209 bp
Sequences
Splice sites
5' ss Seq
CAGGTGTAG
5' ss Score
3.1
3' ss Seq
CTCTCTTTGACATCACTAAGGTT
3' ss Score
4.1
Exon sequences
Seq C1 exon
CCAGACATCCAGATCTCAGAAAACATCACATCATCTTTAAAAAGTGTTTGCGAATGGGGTCAGAAGGTCAATCCTGATGCTGACAGTGATCCTTTACATGCAGATCTACTGTTATATATTACCAG
Seq A exon
GTGTAGCCTCTAAAAGACTCTCACACTACTTAAAACTATTTTTTCAATATTGTACAAACCATTTATTAAAGTCTAGATATTATATTGTGTTATATTATATTATATTCATTTATATTATATAGTAAATATAATTTTCTTACAAACAATTTTTAAAATAATAAATTTTTCTTCATATTTGCTGTTTAGTAACTCTCTTTGACATCACTAAG
Seq C2 exon
GTTTGATCTGGTGTTACCCAATGGGAATAAGCTTGTGAGAGGTGTAACTCAATTTGGTGGAGTCTGTTCCACTCAATGGAACTGTGTTATTACGGAGGATACAGGCTTTGATCTTGGCATCACTATTGCTCATGAGATTGGCCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076270:ENSDART00000146364:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0142114=Reprolysin=FE(19.9=100)
A:
NA
C2:
PF0142114=Reprolysin=FE(23.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGACATCCAGATCTCAGAAAACA
R:
CTGTGGCCAATCTCATGAGCA
Band lengths:
272-481
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]