DreINT0032332 @ danRer10
Intron Retention
Gene
ENSDARG00000017748 | arhgap29b
Description
Rho GTPase activating protein 29b [Source:ZFIN;Acc:ZDB-GENE-031010-44]
Coordinates
chr2:15521050-15522663:+
Coord C1 exon
chr2:15521050-15521187
Coord A exon
chr2:15521188-15522436
Coord C2 exon
chr2:15522437-15522663
Length
1249 bp
Sequences
Splice sites
5' ss Seq
CAGGTGTGG
5' ss Score
6.34
3' ss Seq
AAGAATTATTTGTTTTTCAGTTG
3' ss Score
6.27
Exon sequences
Seq C1 exon
GGCATTTATCGGGTGAATGGGGCGAAGTCTCGTGTGGAAAAGCTGTGCCAGGCCTTTGAGAATGGAAAGGACTTAGTGGAGCTTTCTGACCTCCACCCGCATGACATAAGCAACGTGCTGAAGCTTTACCTGAGACAG
Seq A exon
GTGTGGTCAAACAAACAATCCTAGATTCATCATCTTTATGAAACTTGACATTATAGTTCTCACCCATTTTTTAAGCAGTTTTGGTTTCGGATACTTAAAAAAAATGCACTTCAGCAGGTTCTGACACAATGACAATTAAAAATAAGCCAACATGCAAAAGAATTACATACATTATCGATCAGTGGTCAACAGAATGATTGGCACTGGAATAAAAGAGTTCTTAAGTCTGTTGCGCTTCCAGCAAAAAGTTCTGTATCTTCTACCAGAAGGTAAAAGGAAAAATGGAAAGAGTACATGAGATGGGTTACTTAAAATTCTTTTCACTTCATTCTGAGTGCATTGCTCGTAATATTTGTATACTAGTAAAAGTTTCAGATCCTATTAGTTTTGTAGCAGTTCTGATAATATTAAAAATATTTTAATTTCAGATTTACAGAAAGATTGCCATACCATGTTGATAGATCATCCCTAAAAACACTTGACAAACAAAGCTTGATAAAATAAGTACATGATTTTCTTATCAACACCATAAAACCATAACCTTCGTAGAAAATACAGTCATTAACTACATGGCACAGGAGATTGGTTGCTTTCACCTATGTAGCCATATATTATTGTTTTTTAAAAGATTTATTTATTAATTTTGGCCTTTTTGTCTTTATTAGATAGGACAGTAATGAAACAGGAAACGAAGTGGTAGAGAGAGAGAGGGGGGTAAGGTTGGGAAATGTCCTCATGCAGGCATTCGAACTCGGACTAGATTGCAGCGCTGTAACCACTAGGCTATTGCCCCAACTGAAATATGTTTTTTGACACAGATTAATACTTCAAATATAGTGCAAAATTAACCAAACGCGAAACATTGTCTGTTTACCATAATTTAAATGGTTATATATTTTTTTAAATGTTTTTAAATAATTAATATAAAAGAAATAAATTGGATTTTTTTTTGTGTTGCTCCCTATTCACTTTTTTAGGAATGTGTATGTATATTTCTTCATTGTCAAAATGAATCCAAATGCATTATGAGGGTTAAAACTAAAAAACAACAACAACAACAAAAAAGCATATGCAATCAGAATTTAAAGGGTTTTTTAAAAAATCTGTTAGTCGTTGAGCAAATAAATGGTATTTGTTGTAAATTGGAGAGTTTGATAGCTCATTAAGGGAAAAAATAATACATTTATTAAACCTACCTTGATTGACATTGTCTAACAGGAAAAATAAATAAGAATTATTTGTTTTTCAG
Seq C2 exon
TTGCCAGAGCCGTTGATTCTGTATCGTTATTATAACGATGTCATTGGGCTGGCGAAAGAAACTCAGAACATGGACAAGACAGACTCAGCTAAAGAGAAATCAGCAGGAGAGCAGCTGGGCCTCAGCACTGAGCTGAAAAGAGTGCTCTTCAAAGTCAGAGATCTCCTTCGACAGCTTCCTGCGCCCCACTACAAAACCCTGCAGTTCCTCGTCACACATCTGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017748:ENSDART00000141921:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.197
Domain overlap (PFAM):
C1:
PF0062022=RhoGAP=FE(26.0=100)
A:
NA
C2:
PF0062022=RhoGAP=FE(43.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTTATCGGGTGAATGGGGCG
R:
CGAGGAACTGCAGGGTTTTGT
Band lengths:
346-1595
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]