DreINT0054235 @ danRer10
Intron Retention
Gene
ENSDARG00000013524 | cyp2ae1
Description
cytochrome P450, family 2, subfamily AE, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-090312-107]
Coordinates
chr23:42691137-42691751:+
Coord C1 exon
chr23:42691137-42691297
Coord A exon
chr23:42691298-42691574
Coord C2 exon
chr23:42691575-42691751
Length
277 bp
Sequences
Splice sites
5' ss Seq
CGGGTACGA
5' ss Score
8.06
3' ss Seq
TTTTGTGTGTCTCTTTACAGTTG
3' ss Score
11.93
Exon sequences
Seq C1 exon
GTTTGCCCTTTGACCCTCAGCATCTTGTGACCAACGCTGTGTCTAATATCATCTGCGGGTTGGTATTCGGTCACCGCTTTGAGTACGATGACCATCAGTTTCATCTAATGCAGACGTACATCAATAACATCCTACAGCTTCCCATCTCAAACTGGGGTCGG
Seq A exon
GTACGAGATCAGCCTGCTACCATTTTTATTCATTTTACTATTGGCTTAGTCCCTAATTTATCTGTGGAATGAACCGCCAACTATTCCAGCATATGTTTTACACAGCGGATGCCCTTCCAGCCGCAACCCAGTACTGGGAAACACCAATACACTCTCATTGACACACACTCTCATGCACTACAGCCGATTTAGTTCATCCAAACGTTGCGCATTTCAATTCCACAATGACTTTAAATAGAGAGTCTTGGTGTGTCTTGTTTTGTGTGTCTCTTTACAG
Seq C2 exon
TTGTATAATGAGTTTCCCACCCTGATGTCGCTGCTGCCAGGAAAGCACCAGACGGCGTTCGCCAGCATGTCTAAACTTCAGCCATTCCTAAAGGAGGAAATCACCAAACACCAGCAGGACAGAGAGCCGTCCAGCCCCAGAGACTACATCGACTGCTATCTGGAGGAGATCGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000013524:ENSDART00000035654:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.153
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(11.6=100)
A:
NA
C2:
PF0006717=p450=FE(12.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTGTGACCAACGCTGTGTC
R:
GTCGATGTAGTCTCTGGGGCT
Band lengths:
292-569
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]