DreINT0054250 @ danRer10
Intron Retention
Gene
ENSDARG00000098995 | cyp2k6
Description
cytochrome P450, family 2, subfamily K, polypeptide 6 [Source:ZFIN;Acc:ZDB-GENE-040426-1571]
Coordinates
chr3:12672768-12673364:+
Coord C1 exon
chr3:12672768-12672909
Coord A exon
chr3:12672910-12673179
Coord C2 exon
chr3:12673180-12673364
Length
270 bp
Sequences
Splice sites
5' ss Seq
AAGGTATTT
5' ss Score
7.64
3' ss Seq
GTGGTTCTTCTGAATATAAGCCA
3' ss Score
-0.48
Exon sequences
Seq C1 exon
GAATCTGGCAAAACAGACTCACTGTACAATTCAAACAATTTATATTGTACTGTTAACAACCTGTTCGGGGCTGGTACCGATACTACAGTCACAACATTACGTTGGGGTCTTCTGCTAATGGCCAAATACCCTGAAATACAAG
Seq A exon
GTATTTTTGCATTTACACAGCAAAGATGTAGTCTTCAGTTGTAATTTTAAAAAGTTGCCACAAAACTGCCAAAAAAAATGACAAGACACATTGAATTAAACTATTGCACATTCGTAATGCTTTGTTATGTTGTAAAAATTACTATATACAACTTTTTGATTAAATAGTGTATGGTAAGTTTTTTATATGTGATAATTCTAATATGCAAGTATAATACAAAGTGTCAAAAATAAATGGGGAATTTAATAATGTGGTTCTTCTGAATATAAG
Seq C2 exon
CCAAGGTTCAAGATGAGATTGAACGAGTGATCGGTGGACGTCAGCCAGTTGTAGAGGACAGGAAAAACTTACCATATACAGATGCTGTGATCCATGAAATTCAAAGATTTGCAGACATTTCACCCATCGGTGCCCCCCGTCAGACCACATGCGATGTTCATCTGAACGGATATTTCATCAAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000098995:ENSDART00000170788:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.062 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(10.2=100)
A:
NA
C2:
PF0006717=p450=FE(13.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGACTCACTGTACAATTCAAACA
R:
TCAGATGAACATCGCATGTGGT
Band lengths:
294-564
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]