DreINT0056544 @ danRer10
Intron Retention
Gene
ENSDARG00000014439 | dgkza
Description
diacylglycerol kinase, zeta a [Source:ZFIN;Acc:ZDB-GENE-111104-3]
Coordinates
chr7:38810328-38810642:+
Coord C1 exon
chr7:38810328-38810436
Coord A exon
chr7:38810437-38810546
Coord C2 exon
chr7:38810547-38810642
Length
110 bp
Sequences
Splice sites
5' ss Seq
AGTGTGAGT
5' ss Score
6.96
3' ss Seq
TGTGCTTTGTGTTTGAGAAGGAA
3' ss Score
3.71
Exon sequences
Seq C1 exon
GAAGGCTATTGCCAAGTCCGGTCTCCAGCACCTCTCATGCCAGCCCAGTGCTGCAGTGTTAGCCAAAGGGGAAACGGACAGAGAAATACGCAACACAGTAGACTGGAGT
Seq A exon
GTGAGTTACAAGAGTCATCAAGACTTCTGATCTCCATTCACTGTATTTTTAGAGAGCTACGGTTTCGTTCTTTGGTTTCACGTTTGGTACTGTGCTTTGTGTTTGAGAAG
Seq C2 exon
GAATCAGCTGTATACGGAGAGCACATCTGGTTTGAGACGAATGTCTCGGGGGACTTCTGTTACGTTGGAGAACAGCACTGTTACGCCAAGTCACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014439:ENSDART00000093009:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.027 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0013017=C1_1=PU(44.4=75.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTATTGCCAAGTCCGGTCTC
R:
TGACTTGGCGTAACAGTGCTG
Band lengths:
198-308
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]