DreINT0076120 @ danRer10
Intron Retention
Gene
ENSDARG00000063126 | gusb
Description
glucuronidase, beta [Source:ZFIN;Acc:ZDB-GENE-070705-256]
Coordinates
chr5:1827794-1829873:-
Coord C1 exon
chr5:1829686-1829873
Coord A exon
chr5:1827938-1829685
Coord C2 exon
chr5:1827794-1827937
Length
1748 bp
Sequences
Splice sites
5' ss Seq
GAGGTTAGA
5' ss Score
4.15
3' ss Seq
TTGTTCTGCTGTTTCTGTAGGTT
3' ss Score
10.23
Exon sequences
Seq C1 exon
GACTGGTCAGTTATAATGTGTCAGTGTTGGGCGGCTCTAAGGCGGCTGTGAAAGTGACGCTCTCAGCGAAGGACGGCAGATGCGTGGCCTCGTCTGAAGGCCTCAGTGGAGTCCTCAAAATCACAGACGTCAACCTGTGGTGGCCTTACCTGATGCACGAGAACCCCGGATACCTGTACTCCATGGAG
Seq A exon
GTTAGAAAACTCTCTCAGACACTTCCAAGGGTATTTAAAGGGACAGTACACCCAAAACTGAAATATCTGTCATCATTTACTACCCTTTACTTGTTTCAATCCTTTATGAGTGTCTTTCTCCTGTTGAACACAAAAGAAGATATCGTGAAGAAAGCTTTTTGCTAGCTCGGGTAAATATTATTTAAAAAACTTCAGATTAGTAAATATATAGAAACTGACAGTATTTAGGATTCATCACAAGTAGGCCTGTTCCAATAATCAATATAGGGACTTATTGAACAACACATGGACATGAGCTCAATCAATTCTGGTGATGGAATATATATCACCCATACGTAAAGACAATATCAGCAACATTTGAGCTGATTGTGCATCATCTCTATCTATATATAGTTATTAAACACTGTAGTATTTGCTATAAATGAATGACTACGGTATATTTTCATATGGGTGACAGCTGAAAATAGATCTGGTAGCAGATATCACAGCCTCTGTTACTCTTCAAGAGTTCACACTAAGCTGATAATTGATAATAAAGATAGTTTGGCGTGCTGTCCCGGGAGAGAGCACTGAGCTTATAAGATCCTCGAGCCCTGGGCTCCCACCCGTTGCAAGGCGAGAGGGGAGTTTGAGCTCAGGTAGATCTTGATGGCTCCCCCTCTTGCTTATTGTAGCTAAGTGTCGTATATGGATGATAAGAAGGTGTACTCAGAGTTTGGCTAAAGTATTTTGGATTAATTGTTTAGGGTGGTTGTTTTCTGAACTGTGGAAGGAAACCGGAGGACCAGGGGAAAACCCAAGTGATCACGGGGAGAACGACCAAAACTCCACACAGAAATGCCTCCTGGTTTAGAAAGGAATTAATCCAGGGGCATTCTTGCTGTGAGGCAACAGCACTAATCACTGGCCACCGTGTCGCCCGTTTGAAAAGAGGGAATGTATGGTTGGGTAGGGGGGGTTTCTTCAAGACGGAGATATTGAGATGAGAAAAGTCTGGTTATTTAGAGTGAGTTAAGGATCGTCTGATAGGATAATTAGTCACTAGCTAAAGTTTGAACAGCTGTGAGCAATCATAAGCACGTGATCCTCTCGAAATTTGTTTATAAATAAACTTCACCAACATTTATTATTGTTTATTTAGGAGATGAGTTTTCACATTCTGACTCCAGTGTCTCATTTTTAAGCTGTTCAAATGACAATAATTAAATGAAAAATTCCTAATAATTAAATATGATGTGTTCTCTGGAAGAGTGTACTTGCATTGTAATATTATTTTGTCATTCTGGCATTATGTAATGAGTGATTGTCTTAAAATGACAATAATATTGTTTATCGCAAAATATTTTAGTGCAATATATTGTACGACAAAAAATAGATATCGTGACAGGCCTAATAACAAGTGCTTTGGTTAATTAATACTTCAGTAATACAAAACTACTTCTAATAATAATAAATAAAATGTTTAATAAATGATTTAGTTAAAATAAAATCAGCCACCAACTAAAATTCACTCAAAAACAAAACAAAAAATGATAAATTTTTGTAAATTAAAACTAAAGTTCATTAACTGATTTGCTTCCAGCGTTGTTTAAAAAAAAACAAATGTCTTTTTTCATGTATTAAAATGGTTTGGTTTATAATATATTATAATAAACATAGCTGTTGATTGTTACATGCAAATAAAGGGAAATGAAGTATCTGCTTGAGCAACATGTGATGCCAGTGGTTTTGTTCTGCTGTTTCTGTAG
Seq C2 exon
GTTCAGGCGATCGCAGAAGACGGACAGACGGATATTTACACTCTTCCTGTGGGCGTTCGTACTGTTCAAGTCACCAGATCCAAGTTTCTCATCAACAACAAGCTGTTTTACTTCCACGGGGTCAACAAACACGAGGATGCAGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063126:ENSDART00000091932:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0070316=Glyco_hydro_2=FE(62.6=100)
A:
NA
C2:
PF0070316=Glyco_hydro_2=PD(19.2=39.6),PF0283612=Glyco_hydro_2_C=PU(8.6=54.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGGTCAGTTATAATGTGTCAGTGT
R:
GCATCCTCGTGTTTGTTGACC
Band lengths:
327-2075
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]