MmuINT0075324 @ mm9
Intron Retention
Gene
ENSMUSG00000025534 | Gusb
Description
glucuronidase, beta [Source:MGI Symbol;Acc:MGI:95872]
Coordinates
chr5:130474280-130475398:-
Coord C1 exon
chr5:130475211-130475398
Coord A exon
chr5:130474424-130475210
Coord C2 exon
chr5:130474280-130474423
Length
787 bp
Sequences
Splice sites
5' ss Seq
GAGGTAACG
5' ss Score
9.65
3' ss Seq
TGCCACATCGGCTCTCCTAGGTG
3' ss Score
5.85
Exon sequences
Seq C1 exon
GGCTGGTGACCTACTGGATTTCTGTGCAGGGCAGTGAACATTTCCAGCTAGAAGTGCAACTTTTGGATGAGGGTGGCAAAGTCGTGGCCCATGGGACAGGGAACCAGGGTCAACTTCAGGTTCCCAGTGCCAACCTCTGGTGGCCTTACCTGATGCATGAGCATCCAGCCTACATGTACTCCTTGGAG
Seq A exon
GTAACGGTGATGTGGGACTGGGGCTAAGGAAGGTCCTTTGCTGCCACCTGGTGACAGCAGGCAACCAGGACAGGTGGCTAAATGGGTACTGTCTGAGATTTCCGGCTTTTTCAGTCACTTTGTGGGAGAGTCCAGCTGTGAATTGTGAAGGTCCTGAGATCATGTTCTGGTTAAAGACAGAGACTGTCATTTCTGCATCTCAAGTGCAGGCTTGTGGGATACGTAGGCACCAGGGGTGTTGGAGAATAAGATCAAACGAGCAGTCGCTGAGGTCAGCCGGGTCTCCCTAGTGAATTCCAAGACAGCCAGAGCTCCATAGTGAGACCCTGTCTCAAACAATCAGTGTGTCCCTCCAACCCCCACCTCCCAACAAAACAAATCCCAAAACCAGCCGAGCTGTGGTGGCACATACTTTTAATCCCAGAATTTGGGAGCCAAGGCAGGTGGATCTCTATAAGTTGGAGCCAGCCTTGTCTATGGAGTTCCAGAACATTTAGGGCTACACAGAGAAACCCTGTTTTGCAAACGAAACCAAACCAAACCAAAACATAACCAAAACCAAACAGCAGAGAGATGCCTGCCTGTAATTTTAGCACTTGGGAGGGACAGGCAGGAGAATCAGGAGTTCTAGGTCATCCTTAGCTACAAGTGAGGCCAGCCTGAGCTACATGAGCCTGTATTTAAAAAAAAAAAAAAAAAAAAACTCCAGTGGTGTGGGGTGAGGCCTTCCTGTTGGGTGACGCGCAGTCCACCTGGATGTTCTTGCCTGCCACATCGGCTCTCCTAG
Seq C2 exon
GTGAAGGTGACAACAACTGAGTCTGTGACTGACTACTACACCCTTCCTATCGGGATTCGAACAGTGGCTGTCACAAAGAGCAAGTTCCTCATAAACGGGAAGCCCTTCTATTTCCAAGGGGTCAATAAGCACGAGGATTCAGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025534-Gusb:NM_010368:5
Average complexity
IR-C
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0070316=Glyco_hydro_2=FE(62.6=100)
A:
NA
C2:
PF0070316=Glyco_hydro_2=PD(19.2=39.6),PF0283612=Glyco_hydro_2_C=PU(8.6=54.2)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCTGGTGACCTACTGGATTT
R:
ATCCTCGTGCTTATTGACCCC
Band lengths:
326-1113
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: