DreINT0086503 @ danRer10
Intron Retention
Gene
ENSDARG00000103394 | kif5bb
Description
kinesin family member 5B, b [Source:ZFIN;Acc:ZDB-GENE-070629-4]
Coordinates
chr12:41039839-41040609:+
Coord C1 exon
chr12:41039839-41039984
Coord A exon
chr12:41039985-41040460
Coord C2 exon
chr12:41040461-41040609
Length
476 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGG
5' ss Score
8.41
3' ss Seq
TGTTCTGTTTTCACTGTCAGAGC
3' ss Score
8.88
Exon sequences
Seq C1 exon
ACGTACGTTCCCTACAGAGACAGCAAGATGACCAGGATCTTGCAGGATTCTCTGGGTGGAAACTGCAGGACCACTATAGTGATCTGCTGCTCTCCATCCTCCTTCAACGAGGCCGAGACCAAATCAACACTCATGTTCGGACAGAG
Seq A exon
GTGAGGACATAACCATACACACTCAAAATGAGAGTTTTCAATTGTCCTGTTATGCAATAGTAAGGTGTCCTACAATAGGTTGACATCCAACCAAGGTAAAAAAAAAACAACTTAATTAAATTTTTATCATATTATAACATTTTTGCATTGCACGTCATGAATTTGCCAATTTGTTGCGCCTTTTGATATTGGAATATTTGCAAACATGTACATTTATAAACAGCTAGAGTACACACTATATAAAAGGTAATATCTAAATTAAAAACGTCTGGTTTTTAAGAATGCATTAGTAAATGTTGAATTATGATTAATAAATTCTGTACAGGTATCTTTCCTTTTCAGTTCATGTTAATACATACACTAGCCAACATTAACTGATGGAACCTAAGTGTAAAGGGTGACCAAAAAACATCAAGGGAGCTTTTAATGAAATGTTTGCTTTGAGCGTCAAGTAATTGTTCTGTTTTCACTGTCAG
Seq C2 exon
AGCAAAGACCATCAAGAACACAGTGTCAGTGAATGTGGAGCTGACAGCAGAGCAGTGGAAGAAGAAATATGAGAAGGAGAAAGAGAAGAACAAGACGCTTAAAAACACCATCACCTGGCTGGAGAACGAGCTCAACCGCTGGAGGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000103394:ENSDART00000170976:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.078
Domain overlap (PFAM):
C1:
PF0022518=Kinesin=FE(15.4=100)
A:
NA
C2:
PF0022518=Kinesin=PD(1.3=7.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGTACGTTCCCTACAGAGACA
R:
CATTCCTCCAGCGGTTGAGC
Band lengths:
295-771
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]