GgaINT0101233 @ galGal4
Intron Retention
Gene
ENSGALG00000007215 | KIF5B
Description
kinesin family member 5B [Source:HGNC Symbol;Acc:HGNC:6324]
Coordinates
chr2:14148747-14150334:+
Coord C1 exon
chr2:14148747-14148892
Coord A exon
chr2:14148893-14150185
Coord C2 exon
chr2:14150186-14150334
Length
1293 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
3' ss Seq
AGGTTTTTCTTTCTGTGTAGAGC
3' ss Score
7.46
Exon sequences
Seq C1 exon
ACTTATGTTCCGTATCGTGATAGCAAAATGACCAGAATCCTTCAGGATTCACTAGGGGGTAACTGTAGAACCACTATTGTTATTTGCTGTTCTCCATCTTCATACAACGAATCTGAAACTAAATCTACTCTTCTGTTTGGCCAAAG
Seq A exon
GTGAGTACGTGATTTAAATGATGAGAGAGAGACAAGAAAAAGAATTTCTAAGTGCTGTGTATGCACTACAGTTGCATGCCTAACAATGACTGATAAAACAAACCTTCTACAATAAACAAAGCAGTGAAGCTGTTCTTAAAAATATGTAGTTTGAGATTTTAATTTGATGCTGATTACAGAATTAAATGTCTCTTAATATTTTGCTATTGCTAAACACAGAAATAAAGGCAGAGTGAGAAATAAGTAACCCAGTCAGGATTCCTAAACCAAATTAATCAGAATGTTTGTGGTGTGGAATGATGACTGTGTTCTGCAGCAGTAGAATTCTACTTTGGTCTCCTTTTAGAATCTAGAAGATCCTTTCCTTGCAATATTTCTAGCATTCAGGTATGGGGAAGTGTCTTCAAACTGTAGATTCATTGTGTCTTGCTCTTGATGTCTTCAGGAGTTGTTCAAGTGACTATAGTTGGTGTGGTGTATAACTCAGCTTTCTGGGGTTGTTAAGTTTCTGCTGAAACCCTCAGATGTAGGAACTAAAGGCTAAGTAAATAGTATGTAATTCAGTAGGAAACAATGTGCATTTTGGTAGTAGTCAACTCCCAGTGAGGCTTTGCAGAAATGTGCAGGAGTCATGGCAGCGGCAGGAATGGGTTTCTGTCTACCAATAAGCATTTTTTTTTCCGGTTTTGATCTTTTGGAGGGGATAATTCTTGGTAGGAAGTTGTACATGAGTTACTGATGTAAGCCAGGTTCATCGCTAATGCAACGACTAGATGTTAACTTCATCAAGGTGTATTAGTCCTTGATATTTTGTCTTTGGAGTTAATGCATTTGGATGAGATGTGCTTGCCTGAATGTATCACTTGAAATAAATGCATCTGTATGTTATAGCTCATACTCCTCTTTGGTTAATGCAGATCTAGTCACTGGACTTGGGAGTTGTTCCTTAAGCTAGGTGATTCACATCCTGAAAGTATTATGCTGTCCACTAGAGAAATGTTTGTTGGATATGGTAAATATTAGTCTTTGTTCAAAGCTGTACGCCATCCCAATTCAAGTGAAAGTATACAGCCGTTTCTTTCCAACTCAACTGGCTTGAGATTTTTTTCAGTCTTCTCCATACCTGTTACATTACTGAGTCAATTAACAATTGTATCAATCTAATAGAACAAATAATAAATGGACAGAGTGTAGCGATGAGCTTTTGGAAGAGGTGGGAGTTGTTTACACTTCCAATATTATTTTCTTTTTTAAAAAATAGAGTCTGTTTCAGAGGTTTTTCTTTCTGTGTAG
Seq C2 exon
AGCAAAGACCATTAAGAACACAGTCTGTGTCAATGTTGAGCTCACGGCAGAACAGTGGAAGAAAAAGTATGAGAAAGAAAAAGAGAAAAACAAGACACTGCGTAACACCATACAGTGGCTGGAAAATGAACTCAACAGATGGCGGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000007215:ENSGALT00000011680:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.014 A=NA C2=0.178
Domain overlap (PFAM):
C1:
PF0022518=Kinesin=FE(15.4=100)
A:
NA
C2:
PF0022518=Kinesin=PD(1.3=7.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTTATGTTCCGTATCGTGATAGCA
R:
ATTCCGCCATCTGTTGAGTTCA
Band lengths:
294-1587
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]