MmuINT0087308 @ mm10
Intron Retention
Gene
ENSMUSG00000006740 | Kif5b
Description
kinesin family member 5B [Source:MGI Symbol;Acc:MGI:1098268]
Coordinates
chr18:6222719-6223691:-
Coord C1 exon
chr18:6223546-6223691
Coord A exon
chr18:6222868-6223545
Coord C2 exon
chr18:6222719-6222867
Length
678 bp
Sequences
Splice sites
5' ss Seq
AAGGTTTGT
5' ss Score
7.81
3' ss Seq
TCTGTAATTGCATCACTTAGGGC
3' ss Score
4.57
Exon sequences
Seq C1 exon
ACCTATGTTCCTTATCGAGATAGTAAAATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTCCATCATCATACAATGAGTCTGAGACAAAGTCAACACTCCTCTTTGGTCAAAG
Seq A exon
GTTTGTTGTTGAAATACAAAGTATAAAGACATGGCATTAAAGTTACTAATGTCAGCTACTGCTCAAATCCAGATGTATTTGTCAGAGAACTAGATCTGTACTTTTGAATCAGGACATAGGTCATATAAGTTAGAGATGTATTTTAGACTTAACGAGCTTTAACTTTTCATAGACATTAAAATTGAGAAATGTAATTGTTTAATTTCCTTTTGATGAAAGTAAACATCTGAGTCAGATGATGAAGTAAAAGCTTTTCAGATATATGAGCCTATGTGAAGCTCACTGATGCACTTGAAGTAGCATACATGAAAGAGCTACTTAGGAAAATCAATAACAGCCTTACCTTAGACCCATAGTGATAGGTAACGGATGCTACTGCTTCAGGATTGCAAGTGAGTTGGGAAAATAACTGCATTTTCTTAGTTTTGATTTTTTTTTTTTTTTTTAATGTTACCAAAGACTGAGTGCTGACAGAAATTTATTTGGGTTGTGGATAGATATTTTTTTAGTTTTTGTTCTAAGAAAGAACTAGATAATCTTGGACAATTTGTTATTTAGCTATATCATATATTAAATACGGATGTCAATTAAAAATACTTTCAGTCATATGCTTCTTTTTTCCGTAATGATTACTCTTCGTTAATGATGTCATTTTTTTTCTGTAATTGCATCACTTAG
Seq C2 exon
GGCCAAAACAATTAAGAACACAGTCTGTGTCAATGTAGAGTTAACTGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAAAATAAAACTCTACGGAACACTATTCAGTGGCTGGAAAACGAGCTAAACCGTTGGCGTAACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000006740:ENSMUST00000025083:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.039
Domain overlap (PFAM):
C1:
PF0022518=Kinesin=FE(15.4=100)
A:
NA
C2:
PF0022518=Kinesin=PD(1.3=7.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCTTATCGAGATAGTAAAATGACCA
R:
GTTACGCCAACGGTTTAGCTC
Band lengths:
286-964
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types