DreINT0091880 @ danRer10
Intron Retention
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35465245-35467608:-
Coord C1 exon
chr19:35467591-35467608
Coord A exon
chr19:35465363-35467590
Coord C2 exon
chr19:35465245-35465362
Length
2228 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAG
5' ss Score
9.65
3' ss Seq
TGTATTTTCTCTTTCCTCAGTTC
3' ss Score
9.36
Exon sequences
Seq C1 exon
TTCTTCCTTGGGAATCAG
Seq A exon
GTAAAGTGTGAGCAGTGTTTTTGTGACTTGTCCTCTGAAATCTGTTGCCTCTGCGCAGTTCCCATGAGCGCCCGCTGTAGAAGCTTGTCTTTTATTCTGGGTTTTTTTTCAGACAGCCAGATCTAAATCTGTACACAACAGTTTCTGCATGGGCTTGCTAAGAATTCTCTGTGGACAGGCCACATTCTTGGCTGCTGTCTGAAATGGGGATTTCTTTAGTCCCTTCCTGCTTTCTCAGCGGGCATTTCCTGTCTGGGAGACAAAAATTGCAGTACTATCGCCAAAATATTAAACGCATTTATATATACGCCTTCAGAATCCAGAGGCAGATTTCAAAGGGGTTAGCTTTTGTGTTGTGGTTGGGATGTGCTTGTTTTACTTTATTGAAAATCATTTATCATGATCAGGAAATGGTTCGGATTTACTGCGCTCTCTATTATGATTTTAAGGTTGTTTTGGCTTTTGGTGGTGGAAAACTATCCAACTGCTGATTTCTTGCCTTTGAGAAAATAAACCTTCCACCAAAGCAATTCTTTGAAGTGAAAAAGGACAAACGTCCAGGGAAAAATTCCAGAGAATTGCTGTGGTGTGTGGTGTTTATTGTAGAGATGGGATTTGAGCTGGACATAAATCAAGTAGAATATAAACCAATGTGGTTTGCTGCATGTCGCAGTGTGATTTGCTTTGATTTTCCTGGTGCTTAAGAATGTAATTAGTAGAATTAGCTAATTGTTATATGATTTTAAAGGAATAGTTCACCCATTTTAGTTCTCTTTTTTTCTGTGGAACATAAAAGGAGGCATTTTGGTAACAATTTACAAAAAGGGTGTATTAGTTAATGTTATTTAAAGCATTTACTAGCATGATCAACAATAAACAATACGTTAATTACAGTAATTTTAATTTAACATTTGTTAATGTAAATGAAGTTGGTTAATGTTTTTTCATGTTAACTCACTGTGCATTAACTAATGTTAACAAGCATGAATTTGTATTCATATAATAATAAATTCCATATGAGTATTGGTTATTATTAGCTCATGTTAGTAAATACATTAGCTAACATTAACTAATGAACCATATTGGAAAGTGGGACTGACATTTAAAAGGTGCTGTAAGTTTTTGAGTCTTCTAAAGCATAAAAATAGCATCGTGTGTTGCAGATATTTAAGAAACATGCTAAGTGAACATTCTTGTTTATCTGAAAAAAGCTATATAGTCAGCTATTCTGTTTAGAAAATGTGCATTACATGCTGAAACATCTGTCTTTGTTTTGGTCTTTTAACCCGCCCACTGCAACTTAAGCCAATTATATTTCTCCACCCTGGGTTGCCCTGATGGAAAACAGCGTATTTGATTCATTCATTCAGAAAGGCTCAGTAGCAAAATCCGAAATGAGACGCAGATTCAGAGTTTCACATGAGGGTATTAATTAACAAATAATCTAAATATCATGAATGTAAACAATAGGTGAGCAATAGGTTACATTGTAAGCCCGTGTCCTAACAACATGCTATGTGAGGAGATTTGCAGTGATGAGCAATTCGCACCAGACGAAACACAACAGAAATTTAAATAAAGCCATTCAGAATAGTGCACTTACTGCACTCCAACCAAATGGTAAGGTTTATAATCTAATTAATACATATTAAACCTTTTTTAACACTATTAAATGTAGATGCTGAATCACTGATATGTGTTGGCTCGCAATAAATGTCATGTAAAATGGCATTTAAACTCAAGTATTAGCATTTAACACTGAATAAAGCACATGAGGTGTACCTGAGCTAATCATTGTCTTAGTTCTGTTGTTCACCTGTGAGGAATAGAAGCCGTTTCTAATATATCAATTCGAGATGTGGGTTACAACAAAAACTCCTTTTATTATGGAAAATATTCCTTCTATAGCATACCGTTGCTTTTAGTTAGAACACGGTTTAAACCAGCCTTTAGGCTCGATAGTCAGACTAGCTTCTGTTGGTCCTCAATCTGGCAACCTGCGCTTGCGTGTGTTTTGATCCAGGAATGCAACACCTTCTTCAACCACTGGGTCTCAAACTTACATACTGCACCTTTAAGAAACTGTCATGTATATTTCTTCAGAATTTCTCCTTTTTTCCACATAAATACATTTATTGGGTGAACTATACCTTTTAACGACATTATTAAATTCTCATCTTTTTTTGTTTGTTTGTCCTCTTCCTTTTCTGTATTTTCTCTTTCCTCAG
Seq C2 exon
TTCGGAGACTCACAGCAGCTTCGTCTCGTACGAATTCTGCGAAGTACAGTCATGGTTAGGGTTGGAGGAGGCTGGATGGCGCTGGATGAGTTCCTGGTGAAGAACGACCCTTGTCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533:ENSDART00000146394:92
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.001
Domain overlap (PFAM):
C1:
PF0218712=GAS2=FE(6.3=100)
A:
NA
C2:
PF0218712=GAS2=PU(87.5=96.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]