DreINT0094993 @ danRer10
Intron Retention
Gene
ENSDARG00000017229 | megf10
Description
multiple EGF-like-domains 10 [Source:ZFIN;Acc:ZDB-GENE-080506-1]
Coordinates
chr10:16150856-16151354:+
Coord C1 exon
chr10:16150856-16151102
Coord A exon
chr10:16151103-16151236
Coord C2 exon
chr10:16151237-16151354
Length
134 bp
Sequences
Splice sites
5' ss Seq
ATTGTAAGT
5' ss Score
8.54
3' ss Seq
GTTTTGGTGTCTCTGTGCAGCTG
3' ss Score
9.22
Exon sequences
Seq C1 exon
CGTGTGACCGTGATCACTGGGGCCCACACTGCAGTAGCCGGTGTCAGTGTAAGAACGAGGCGCTGTGTAACCCCATCACTGGAGCGTGCATATGTGCACCCGGGTACCATGGCTGGCGATGTGAAGACCTCTGTGACCATTCCACTTATGGCAACAATTGCCAACAGAAATGTCTGTGCCAGAATAATGCTACATGCCACCACATCACAGGCGAATGCGTGTGCAGTCCAGGATACACAGGAGCATT
Seq A exon
GTAAGTTTACCTGCATTTTTTTTTGCAAACAATAGAAGATGGTGTTGGAAATACCGTGGCCATGGGAACTAAACCGATACCTTTAATTTAAGTTAGACTGGTAAATGCATAAATGTTTTGGTGTCTCTGTGCAG
Seq C2 exon
CTGTGAGGACTTGTGCCCTCCGGGAAAACATGGACAGCAATGTGAGGAGCGTTGTCCCTGTCAGAACGGTGGTGTGTGTCATCATGTGACTGGAGAATGTTCCTGTCCTGCTGGTTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017229:ENSDART00000043936:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=WD(100=55.4),PF126612=hEGF=PU(84.6=13.3)
A:
NA
C2:
PF126612=hEGF=PD(7.7=2.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGTGTAACCCCATCACTGG
R:
CACCGTTCTGACAGGGACAAC
Band lengths:
257-391
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]