MmuINT0097625 @ mm10
Intron Retention
Gene
ENSMUSG00000024593 | Megf10
Description
multiple EGF-like-domains 10 [Source:MGI Symbol;Acc:MGI:2685177]
Coordinates
chr18:57240544-57242783:+
Coord C1 exon
chr18:57240544-57240790
Coord A exon
chr18:57240791-57242662
Coord C2 exon
chr18:57242663-57242783
Length
1872 bp
Sequences
Splice sites
5' ss Seq
CTTGTAAGT
5' ss Score
8
3' ss Seq
GTTTCACTTAAATCCCTCAGCTG
3' ss Score
4.1
Exon sequences
Seq C1 exon
CTTGTGATGGTGATCACTGGGGGCCTCACTGCAGCAGCCGATGCCAGTGCAAAAACAGAGCTTTGTGTAACCCCATCACCGGTGCTTGCCACTGCGCTGCGGGCTACCGGGGATGGCGCTGCGAGGACCGTTGTGAACAGGGCACGTACGGTAACGACTGTCACCAAAGATGCCAGTGTCAGAATGGAGCGACCTGTGACCACATCACTGGGGAATGCCGTTGTTCACCTGGGTACACTGGAGCCTT
Seq A exon
GTAAGTTGGTGCGCTGTGCAGTCGCACCGCAAATACTGTGTGCCTTGACTTGCGCTGCTGCCTCAATTGCTATTGCTGGGTTGAGGGTCCAGGCTCCTCACAGATGGAGGGACCAGCTGAGATGGCCACTAGGGTCCCCTGCTGATTGACTTTCGAATGCTAAAGCTCAGCCAAGATGTGAGGACCAGCCTCAGGTTCATCGGATGGTGTGCGAATGAGGGTATTTGGGGGTTACCGTGGCAGGCGTGGATTTCTGTTGAAAATCTCCCACAATTCCATCACTGTCTAGGAATAGTTAATAGTGGTCCAGGTGGCTCTGCCCTTGTGGTAATTAAGTAGATATATCCGGGTGGATCCAGAGCCCAAGAAAGAAGACTGAAATGTTTGCATGGAATGAAAGTCAAGAACGTGAAAGGTCATTGGAGTCATTGGTGCTCATGGTCACACATATGTCATTATGCTCAGAAAAGTGCACATATCGCATCCACTAGGATGTATTGCACAGGTTTATAATAATAATATTAGTATATAATAAAATAATTATTAGTAGTAACATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATAGAACACTGAAGTAACTCCTTAATCTCCATGACATTATTTTGGGAAGTGCATTCTTTGGCTTGCTCCCCTTAACAGAGTTTTTAAAATGCAAAGTTTGGCATGAAAAAAAAAGAAGTAAGAGAGAAAAATCTTCTTCCCTTACTGAGAAAATTATTCTCGTAAATCACAGAACTAATAGTTGTGGAAATGTAGCTGATTAGGTAAATATGTCCTGCTGACAGTGCTAATGTCTATAAAGTAGAGCTAAAGGAAAAACAAAACAAAGCAAAACAAAAAACCGCACTAGCCTAGAAAACCCCAGTTTTCAGAAGAATAGATTAGCGTCTCCAACTGCTTAATTGTTAATATCGCTATTGTGGGTTTTCATCAAGGTCAACATTACACAGGGCTGTAGGAAGCTGGGAAGCATTTGATCAGAGCAAAACTCGGGTGGTTTAGTGGGGAAGTTAGAAGCGCAGTGGAGCTAAAGGAGTGGGGGCTTTTCATTCAAAAGAAGAGGGTGCTGAAAACTGTGACCAAGCACACAATGGGAGCATGTGGCCATTTAGACCTAGGCTGTCTTTCAAGTTAGAACAAACTTCCAAAGGAAAACAGCTTGAGCTCCAGCTCAGCTTTGTAAGCCGTCCAGGGAAAGGATGCTCTTCTTCCTGGAAGTCTAGAACAGGGGCGCTTTCCCTCCACTTGGAAGTTCCCTGGATGGATGGTGGGGTTCATGCAATGCAAGTCTAAGGATCTGCGTTTGGATCTCCAGCACCCACGTAAAGAGTCGGTGCAGTCTTTGGTGCCTTTAACCCTGGTGCTGGGAACCAAGGGAGGGAAGAGACAGAGACAGGATCCCTGGGGCTTGCTGGAAACCATCTAAGCTATAGTCAGTAAACTCCAAGCTCAGTGAGAGACCCCAAGGGAATAGGTTGGAAACTGGTACAGGACACCCTACATCCTCCTCTGGCCTCCACATACATACATATGGGTACTACACACACACACACACACACACACACACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATATGCAGGGAGAGAGCCCAAACCAAACCAAGGACCCAGTGGTCCATTAGGACATTTCCATCCACTCTGATTAATGTTCTGATTAAATTAGTAATTACTGAAGGAGGCAAGGTTGTATATTTCAGGGTTCTGGATGACATCATGTCTGTGACTCAGAGGATCCTGTACCTGGATAAATAATAGTATCTTTTGTTTCACTTAAATCCCTCAG
Seq C2 exon
CTGTGAGGATCTTTGTCCTCCTGGCAAACATGGTCCACATTGTGAGCAGAGGTGTCCCTGCCAAAATGGGGGTGTGTGCCACCATGTCACTGGAGAGTGCTCTTGCCCTTCTGGTTGGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024593:ENSMUST00000075770:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=WD(100=55.4),PF126612=hEGF=PU(84.6=13.3)
A:
NA
C2:
PF126612=hEGF=PD(7.7=2.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATCACTGGGGGCCTCACT
R:
CATCCAACCAGAAGGGCAAGA
Band lengths:
357-2229
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types