RnoINT0091327 @ rn6
Intron Retention
Gene
ENSRNOG00000013674 | Megf10
Description
multiple EGF-like domains 10 [Source:RGD Symbol;Acc:735084]
Coordinates
chr18:52305466-52307576:+
Coord C1 exon
chr18:52305466-52305712
Coord A exon
chr18:52305713-52307455
Coord C2 exon
chr18:52307456-52307576
Length
1743 bp
Sequences
Splice sites
5' ss Seq
CTTGTAAGT
5' ss Score
8
3' ss Seq
GTTTCACTTAAATCCCTCAGCTG
3' ss Score
4.1
Exon sequences
Seq C1 exon
CTTGTGACGGTGATCACTGGGGACCTCACTGCAGCAGCCGGTGTCAGTGCAAAAACAGAGCTTTGTGTAACCCCATCACCGGAGCTTGTCACTGCGCCGCGGGCTACCGGGGATGGCGCTGTGAGGACCGTTGTGAACAGGGCACCTACGGTAACGACTGTCATCAAAGGTGCCAGTGTCAGAATGGAGCGACCTGTGACCACATCACTGGGGAATGTCGTTGTTCACCTGGATACACTGGAGCCTT
Seq A exon
GTAAGTTGTGTGGAGTTGTAACTCAGATACTGTGTGCCTTGATTTGCGTTCCGGCCCCAATGGCCACTGCTGGGTTCAGGGTCCAGGCACTTCACAGATGGAGAGACCAGTTGTGCTAACCACTAGGCCCCCCTGCTGCTTGACTTTTGAATGCTCAGCCAAGATGTAAGGCCCACCTCGAGGCTCACCAGACTGTGTATGAATGGGGTTATTTGGAGGTTATGGTGGACTTCTATTGAAAATCTCCCACAATTCCATCACTGTCTAGGAATAGTTAATAGTGGTCCAGGAGGCTCTTCCCTTGTGGTAATTAACTGGATATATCCGGGTGGATCCAGAGCCTGAGAAGGAAGACTGAAATGTTTCCATAGAATGCAGCCGATAGTGTGAAAGGTCATCTGGGTCATTGGTACTCACGGTCACACATAATGCATTGTGCACATAAAAGTGCACACATCACATTTACTAGGAGGTTTATAATGATATTAGTATATAAAATAGTAATAATTGATAGTATATTATTATTATTACTATTACTATTATTATTATTACTGGAGAACACTGAAGTCTCTATGACTTTATTTTAGGAAGGGCATTTTTGGCTTGCTTCCGTTAACAGAATTTTTAATTTGGCAGAAAAAGTAAGAGATAGAAAAACCTTCTTCCCTTAACTGAGAAAATTATACTTGTAAATCAGAGAACTAATAGTTGTGGAAATGTAGCTGATTAGGTAAATACGTCCTGCTGACAGTGCTAATGTCTGTAAAGTAGAGCTAAAGAAAAAAACAAAAACAAAAACCTGTGCTAGCCTAGAAAGCCCCAATTTTCAGAAGAATAGATTATTGTCTCAAAGTGCTTAATTGTTAATATCACTATTAAGGGTTTTCATCAAGGCCAACATTAGACAGGGCTGTGGGAAGCTTAGAGGCATTTAATCAGAGCCAAACCAAGGTGGTTTAGTGGGGAAGGTCAAAGGACTGTGGGGTTAAAGGAGTGGGGCTTCTTGTTCAAAAGAAGAGGGTTCTGAGAACTGATTGTGACTGTGCCCACAATGGGAGCATGCGGCCATTTAGATTGCCTTTCCCTTTAGAAACTTCCAAAGGAAAGTAGTTCGCACAGCAGCTCAAGAGCTTTGGGAGACGTCCGGGAAAGGACACTTCTTGTCCTGCAGTTCTAGAGCAGGAGAGCTTTCCCCCACTTGGAAATGACCTGAATGGAGGGTGGGAGTTCATGCTGTGCAAGCCAAGGCTCTGAGTTTGGGTCTCTAGCACCCACATACAAAGCCAGGTGTGGTCTTGGGTGCCTGTAACCCTGGTTCTGGGAACCAAGGGAGGGAAGAGACAGAGGCTCCCTGGGGATTATTGGGCGCCACCTAAGTTATAGTCAGTGAGCTCCAAGTTCAGTGAGAGACTCCATCTAAGGGAATAAGGCGAAGACTGGTAGAGGACATCCTACATCTTCCTCTGGCCTCCACATACACACATATGGGTGCACATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCAAACCAAAACAAGGACCCAATGGCCCATCAGGACGTTTCCAGTCACTCTTATTAATGTTCTGATTAAATTAATAATTGCTGAATGAGAAGAGGTTGTATATTCTAGGGTTCTGGATGATATTACGTCTGTGAATGTGAGGATCCTGTACCAGGATAAACAATAGTGTCCTTTGTTTCACTTAAATCCCTCAG
Seq C2 exon
CTGTGAGGATCTGTGTCCTCCTGGCAAACATGGCCCACAGTGTGAGCAGAGATGTCCCTGCCAAAATGGAGGCGTGTGCCATCATGTCACTGGAGAGTGCTCCTGCCCTTCTGGCTGGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013674:ENSRNOT00000037901:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=WD(100=55.4),PF126612=hEGF=PU(84.6=13.3)
A:
NA
C2:
PF126612=hEGF=PD(7.7=2.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTGTGACGGTGATCACTGGG
R:
CTCTCCAGTGACATGATGGCA
Band lengths:
344-2087
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]