DreINT0099813 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36548371-36548803:-
Coord C1 exon
chr3:36548591-36548803
Coord A exon
chr3:36548516-36548590
Coord C2 exon
chr3:36548371-36548515
Length
75 bp
Sequences
Splice sites
5' ss Seq
AAGGTGATT
5' ss Score
5.88
3' ss Seq
ACTTTGTGTATGTTGAGCAGGGC
3' ss Score
3.98
Exon sequences
Seq C1 exon
CGTGGAAGCAGAATCAACCCAGAAGAGCAATGCTCTGAAAAAGATTCACGAAATGGAAGGACTTCTCTCAGAGCTGCAAGATGAGCTTGAAGCTGAACAGGGGGCTGGTAGGAAGTCAGAAAAGGCCAGAAAGGAGTTGGAAGAAGAGTTGTCAGCCTTGAGGACTGAACTAGAGGACTCATTAGACACCACAGCCGTGCAGCAAGAGCTAAG
Seq A exon
GTGATTACTTCAATAGCATTAACATTTCAGCAACATGATTCCTCCTATACTATCGACTTTGTGTATGTTGAGCAG
Seq C2 exon
GGCTAAGAGGGAGCAGGAGGTGGCTATGTTGAAGAAGTTGATAGAAGATGAAGGCCGAAGCCATGAGGCTCAAGTGCATGAGCTGAAACAAAAACATGCTCAGGCTGTAGATGAACTTAGTCAGCAGCTAGACCAATCTAAACGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:24
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.898
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PD(7.1=26.4),PF045827=Reo_sigmaC=PD(33.3=65.3),PF0157614=Myosin_tail_1=FE(8.3=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(5.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGACTTCTCTCAGAGCTGCA
R:
CCACCTCCTGCTCCCTCTTAG
Band lengths:
179-254
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]