DreINT0099845 @ danRer10
Intron Retention
Gene
ENSDARG00000073732 | myh14
Description
myosin, heavy chain 14, non-muscle [Source:ZFIN;Acc:ZDB-GENE-100921-1]
Coordinates
chr16:16998043-16998846:+
Coord C1 exon
chr16:16998043-16998203
Coord A exon
chr16:16998204-16998737
Coord C2 exon
chr16:16998738-16998846
Length
534 bp
Sequences
Splice sites
5' ss Seq
AAAGTAAAT
5' ss Score
4.06
3' ss Seq
TTAACGATTATGTTTTTCAGGGC
3' ss Score
7.57
Exon sequences
Seq C1 exon
ATCAGTGCTTTAGAGTTGGACAAGAATCTTTTTCGGGTGGGACAGAGTAAGGTGTTCTTCAGGGCTGGAGTTTTAGCTCATCTGGAGGAAGAACGAGACTTGAAGATCACTGACACCATTATCCGCTTCCAGAGCGCAGCTCGTGGATACCTTGCTAGAAA
Seq A exon
GTAAATTCCTATGCATGTTTGGTAGATATGAGGGATGTATTATGTGTACATGTGTACAGTGCTCAGCATAATTGAGTACAGCCAATCTTGAAATTTTTTTATTCATTTTTCAGTGAATGTACACAATGCATTTTGATGCATTTAAACAAAACACATTTATTGAACAGATATACTTATTAACCTAATATTTTAGTCACCAAACATATTGAGAAATTAAAATTAAATTCAAGCAAAATCTTGCAAAAAAAAAAAGACTATTTTAACTAAATTATTACATTGTATGCTTCTCTTGATATCTCCTCGTTTTAAATTTGTATTTGATATTTTTCTATAACATTAATTTGGTTGTACTAGTTTTTTGACCGTTATCGTATTGTTAGATAAGATCCAGATTTGGCTTCAGTACTGCCTAATCTAATGTATATGCATAAATATAATATTGTATAGCTTCCTATTAAAATATGAATTTAAAAGGTTTGTGAGGGGTGTACTTATATATGCTGAGAACCGTATTTTAACGATTATGTTTTTCAG
Seq C2 exon
GGCATTCCATAAGAAGCAGCAGCAGCTTAGTGCTCTGCGGGTGATGCAGAGAAATTGTGCTGCATATCTGAAGCTCAGAAACTGGCAGTGGTGGAGACTCTTCACCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000073732:ENSDART00000142155:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(2.7=37.0),PF0061222=IQ=PU(81.0=31.5)
A:
NA
C2:
PF0061222=IQ=PD(14.3=8.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCAGTGCTTTAGAGTTGGACA
R:
CTTGGTGAAGAGTCTCCACCAC
Band lengths:
270-804
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]