DreINT0099909 @ danRer10
Intron Retention
Gene
ENSDARG00000076075 | myh7ba
Description
myosin, heavy chain 7B, cardiac muscle, beta a [Source:ZFIN;Acc:ZDB-GENE-100914-2]
Coordinates
chr11:24969974-24970341:-
Coord C1 exon
chr11:24970249-24970341
Coord A exon
chr11:24970038-24970248
Coord C2 exon
chr11:24969974-24970037
Length
211 bp
Sequences
Splice sites
5' ss Seq
TTTGTGAGT
5' ss Score
5.91
3' ss Seq
ATTTGCTTAAATCCCACTAGGGT
3' ss Score
7.34
Exon sequences
Seq C1 exon
GGAACTTTAGAGGATCAGATCATTGAAGCTAATCCTGCAATGGAGGCTTTTGGAAATGCCAAGACACTGAGGAACGACAACTCGTCCCGTTTT
Seq A exon
GTGAGTATTTGACACTATAAAAATCATCATTGTTTTTTAATAAATTTTTCTAACATACTGTATACATACAGACTGATAATACTATTCTGTAAAATAAAAAATATCTGCATATTGCTAGCAAGCATGGTTGTTTCAGTCATGATGTTCTAGTTTATGATTAATATGCAAATTAATTGTCCTTCTTGCTTTGTATTTGCTTAAATCCCACTAG
Seq C2 exon
GGTAAGTTCATCAGGATCCATTTTGGACCAACAGGGAAACTGGCCTCTGCTGACATTGACATAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076075:ENSDART00000097316:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.148 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.4=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(3.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAACTTTAGAGGATCAGATCATTGA
R:
TATGTCAATGTCAGCAGAGGCC
Band lengths:
156-367
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]