DreINT0099932 @ danRer10
Intron Retention
Gene
ENSDARG00000035322 | myh7bb
Description
myosin, heavy chain 7B, cardiac muscle, beta b [Source:ZFIN;Acc:ZDB-GENE-090311-5]
Coordinates
chr23:18796960-18797955:+
Coord C1 exon
chr23:18796960-18797063
Coord A exon
chr23:18797064-18797811
Coord C2 exon
chr23:18797812-18797955
Length
748 bp
Sequences
Splice sites
5' ss Seq
AGGGTCAGT
5' ss Score
5.85
3' ss Seq
CTTTAATCTAATCCTGACAGATG
3' ss Score
7.25
Exon sequences
Seq C1 exon
GGAAGAAGCGTGTTTGGATTCCAGATGAGAAGGAAGCATACATTGAGGTGGAGATCAAAGATACAGATGGAGACAAAGTCATGGTGGAGACCAAAGATGGAAGG
Seq A exon
GTCAGTAACGCTGAAATCACTCTTGTGCATTTCTGAGACTACAAAATTATATATATATATATATATATATATATATATATATATATATATATATATATATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATAATTAGTTTACACTATATATGCTTCAGAAGACTGAATTACATAAGACTTGCTTTAAAGAAAAAAATTAATCTTTCTTACTTCCTGTCTTACAATTTAGTAATTTCTTCTTAAGCAACCATGTAATAATATCTATTATAGTTTGATTAAAATGCAAACAAAAAAGTACTGTAACACAATGTTCTAATGTGGATAACATTAATTTAGAATTCTCAAAACATTTGTTAAAATATTTAAAAATCTCAATTGTTTCATTACATACAATTTACAAATACATAAAAAGTTGCCTTTGATAATGTTAAAAACAACATTAAATCTTTTTTGATAAATAATAACACTAACTAATGTAAAAAAGTAACCTTATTGTAAAATGTTATTAAAAATACAGATTAAATAATTTGTGAATACAGATTAAAGGTAATTTACTTAATTATTAGTGAATATGAAAATATTAATGTTACCTTGTAAGATTTATTATATTGTACTGCATTTATATTCAGACTTTACAAGCAGACTTGAATGTACAATCATTCTGTGGCTGAAAAGCACATCATGAAGTAGTCATTCAATTCTTTAATCTAATCCTGACAG
Seq C2 exon
ATGTTAACAGTGAAAGAGGAAGATATTCAGCAGATGAATCCGCCAAAGTTTGACTTGATGGAAGACATGGCCATGCTCACGCACCTCAATGAGGCCTCGGTTCTTTTCAACCTCAGCCGTCGCTACAGTTTTTGGATGATCTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035322:ENSDART00000137438:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.062
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(78.6=94.3)
A:
NA
C2:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(22.8=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAGAAGCGTGTTTGGATTCC
R:
AGATCATCCAAAAACTGTAGCGAC
Band lengths:
246-994
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]