DreINT0100826 @ danRer10
Intron Retention
Gene
ENSDARG00000061635 | myo5aa
Description
myosin VAa [Source:ZFIN;Acc:ZDB-GENE-041027-2]
Coordinates
chr18:39010491-39010771:+
Coord C1 exon
chr18:39010491-39010577
Coord A exon
chr18:39010578-39010662
Coord C2 exon
chr18:39010663-39010771
Length
85 bp
Sequences
Splice sites
5' ss Seq
GAGGTACAC
5' ss Score
5.99
3' ss Seq
CAACTGCTTTCGTTCGTCAGGTG
3' ss Score
7.59
Exon sequences
Seq C1 exon
GTTTGACCCCAAGAGGGCAGTGCAGCAACTGCGTGCTTGTGGAGTTTTGGAAACAATCCGCATCTCTGCAGCTGGTTTCCCATCGAG
Seq A exon
GTACACTGAAGAACTATACATAACCCTACCTCAATTCCTTTTGTCACACTTTCTGACCATTTGTGCAACTGCTTTCGTTCGTCAG
Seq C2 exon
GTGGACGTATCAAGAGTTCTTTAGCAGGTATCGTGTACTGATGAAACAAAAAGATGTTCTGACGGACAAGAAGATGACCTGTAAGAATGTTCTGGAGAAACTGGTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061635:ENSDART00000133618:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.3=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCGTGCTTGTGGAGTTTTG
R:
TGCACCAGTTTCTCCAGAACA
Band lengths:
167-252
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]