DreINT0100857 @ danRer10
Intron Retention
Gene
ENSDARG00000074622 | myo5ab
Description
myosin VAb [Source:ZFIN;Acc:ZDB-GENE-050411-72]
Coordinates
chr25:7463805-7464252:-
Coord C1 exon
chr25:7464146-7464252
Coord A exon
chr25:7464071-7464145
Coord C2 exon
chr25:7463805-7464070
Length
75 bp
Sequences
Splice sites
5' ss Seq
TCTGTAAGC
5' ss Score
4.33
3' ss Seq
TACCATTTCATCACACCCAGGAT
3' ss Score
5.38
Exon sequences
Seq C1 exon
GAATTACCGAGGCACACCAAATGGGTTTATTCCAAATCCTGTCAGCCATCCTAGCCATGTTTTGTGACCTGGCGAAAGAGAGAGGATCATCCAGCTGTAGCATCTCT
Seq A exon
GTAAGCTATCATAAAAATTCAAGAATGAATAATTTCTACTATACTGATTTCACAATACCATTTCATCACACCCAG
Seq C2 exon
GATGAAAACGGACACCTAGCCATGTTTTGTGACCTGACTGAAGTGTCAAATGAATCCATGGCTCACTGGTTGTGTCATAAAAAGCTGAAAACGGCCACGGAAACCCTTAATAAACCTGTGACCCGACTGGAAGCTGTGAACGGTCGGGACGCTCTTGCAAAGCACATTTACGCCAAGCTCTTCTCGTGGATCGTCAGCCAGGTCAACAAGGCTTTGAGCACTTCTTCTAAACCGCACTCGTTCATCGGTGTCCTAGACATCTATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074622:ENSDART00000089034:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.2=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(13.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTGACCTGGCGAAAGAGAGA
R:
TCCAGTCGGGTCACAGGTTTA
Band lengths:
176-251
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]