DreINT0100905 @ danRer10
Intron Retention
Gene
ENSDARG00000062003 | myo5b
Description
myosin VB [Source:ZFIN;Acc:ZDB-GENE-031219-7]
Coordinates
chr21:22414602-22414941:-
Coord C1 exon
chr21:22414767-22414941
Coord A exon
chr21:22414683-22414766
Coord C2 exon
chr21:22414602-22414682
Length
84 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAC
5' ss Score
7.29
3' ss Seq
TCAATTTTGCTCTATCTCAGATT
3' ss Score
8.53
Exon sequences
Seq C1 exon
GTACAACATCAGTCAGCTGGAGGAGTGGCTAAGAGGGAAGAACCTGCACCAGTGTGGAGCTGTGGCCACTATGGAGCCGGTCATTCAGGCCGCGCAGCTGCTGCAGGTGAAGAAGAAGACATCACAGGACGCTGAAGCCATCTGCTCGCTCTGCACTGCACTCAGTTTACAGCAG
Seq A exon
GTACACATTTAGCTTTCATTTTTTTCCTTTCTATTTGTTTTGTTTTGTTTTTACTTTTATTAACTCAATTTTGCTCTATCTCAG
Seq C2 exon
ATTGTGAAGATTCTGAACTTGTACACACCACTCAATGAGTTTGAGGAGCGAGTCACTGTTTCCTTCATAAGAGACATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062003:ENSDART00000166835:38
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=FE(54.7=100)
A:
NA
C2:
PF0184314=DIL=PD(12.3=48.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCACTATGGAGCCGGTCATTC
R:
AGGAAACAGTGACTCGCTCCT
Band lengths:
174-258
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]