MmuINT0104460 @ mm10
Intron Retention
Gene
ENSMUSG00000025885 | Myo5b
Description
myosin VB [Source:MGI Symbol;Acc:MGI:106598]
Coordinates
chr18:74760850-74762656:+
Coord C1 exon
chr18:74760850-74761024
Coord A exon
chr18:74761025-74762575
Coord C2 exon
chr18:74762576-74762656
Length
1551 bp
Sequences
Splice sites
5' ss Seq
CAGGTACGG
5' ss Score
10.88
3' ss Seq
CTTTGCTTTCATTCCAACAGATT
3' ss Score
11.18
Exon sequences
Seq C1 exon
GTACAACATAAGTCAACTGGAAGAGTGGCTTCGGGGCAAAAACCTTCACCAAAGTGGAGCAGTTCAGACCATGGAGCCCCTCATCCAGGCAGCCCAGCTCCTCCAGCTGAAGAAGAAAACCCACGAGGATGCTGAGGCCATCTGCTCTCTGTGCACCTCCCTCAGCACCCAGCAG
Seq A exon
GTACGGCGGTTCAAGACCAGCGCTGGCCCTTCCTGTCTGTCCTTGCCTGCCGGCAGTTTTCTAAGGGGTGCACTTCCAGCCCTGATGACACAGTTCAGACTTGCAACTGATAAGATGAACCAGAGATCGCTTTGGTTGTAAAACCCAACGTCAGTTCCTTGTAACTTTCTGAGCCTTCTGCTTTTGTTTTTTTTTTTTTTTAATTATTATTTTATATAAATGAGTACACTGTTGCTGTCTTCAGACACACCAGATGAGGGCATCAGATCCCATTACAGATTGTTATGAGCCACCATGTGGTTGATGGGAATTGAACTCAGGACCTCTGGAAGAGCAGTCAGTGCTCTTAACCACTGAGGCATCTCTCCAGCCCCTTTTTGATCCTTCTTGTGTGCTAAGTTACAAGACCCACAAACCTTGATAACAAAATAACATGTGCAATGATGATGATGATGATGTGTGTGTGTGTGTGTGTGTAGGTTAGAGGTTGACTGGGAGGAGGAGGTTATCCTACTATGTGGGCTCTGGGATTGACCTCAGATAACCAGCCATGGTTGCCGGAGTCCTTCCCCAGTGAGCCATCTTGCTTGCCTCATGTCACACCTTTTTAAAAACAATTCCAGATAGTTCCTTACCTAGAATTTTTTTCTGAATCTATCAACATGAAGCTGATTGCTTTCTTTATATATTGTCTTTACAACTGTAATTTTTATTTGGCCATATTTTAAGACTCCATTTATATCAGTTTTAAATAAAGCCTTAGCAATCTAACGGTACTTAAATATTTTAAATTCCTCTCCCCATAATTGTATTTATATAGTATAATTTGATTGTAGACATATAACTGGTCTATAAAGAGATAAGCCCTAGATCTTGTCTTTGATCACAACTTCTTAGAAATTGGTCACAGATACCAATAAATAGGAACTAGACTCTCCCAATCGAATGCATATGTTTCCCAACAAATACCTTTGCAACCAACCAAACTCAGTAAGGCGAGGGGCATATGTTTCTCCTCCCTGCCGACTCCTGGGACGAACCTGCAAACTCTTAAGAACTGCACCTTCTTAGTTTTCCAGCTCCCCGCCCCCCAGTAAATGTCTCACCTCTGTGAGCTTGCTTACTGCATGTCCCTGGCCTTGCACACAACCAAAATAGTTGAGGGGCACACAAAAGACTGTGTCTGGTTAAGAAGAAAACTCTTACTTGTAAATGAATTTCCTTAAGGAAGAGGGTGGGAGATGGTGGTTAGGTGCAGTTAAGAAAGCCCTCTCTCATACAAGATCCCTGGTTCTCTCCTGAAGAACCTTAAGCCACCGGCAGGAAAGAGGCCGGGCCTTACAGATAGGAGTTTTCTGAACTCATATATAAGCCACCAAGCCTAGGGAAAGGGTGGGCATCGAGGACATGAGCATCAGACAGGAGCTCACGCCTCACAGGGTCGGCCATGGGCCTGACCACACACCTTGAGGGGTCTGTCCAGAGTCTTAAAAGTCAAAAGTGTCTTCAGTTTATCTAGTTCTGTACTGAGCTTTGCTTTCATTCCAACAG
Seq C2 exon
ATTGTCAAAATTTTAAACCTCTACACTCCCTTGAATGAATTTGAGGAACGGGTCACAGTGTCCTTCATACGAACAATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025885:ENSMUST00000074157:37
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=FE(54.7=100)
A:
NA
C2:
PF0184314=DIL=PD(12.3=48.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAACATAAGTCAACTGGAAGAGTGG
R:
CTGGATTGTTCGTATGAAGGACACT
Band lengths:
254-1805
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types