DreINT0101039 @ danRer10
Intron Retention
Gene
ENSDARG00000099871 | myo7aa
Description
myosin VIIAa [Source:ZFIN;Acc:ZDB-GENE-020709-1]
Coordinates
chr18:3154597-3156318:+
Coord C1 exon
chr18:3154597-3154824
Coord A exon
chr18:3154825-3156150
Coord C2 exon
chr18:3156151-3156318
Length
1326 bp
Sequences
Splice sites
5' ss Seq
CGGGTATGA
5' ss Score
7.63
3' ss Seq
TGTGTGTTTTCCGGGTGAAGGAG
3' ss Score
4.67
Exon sequences
Seq C1 exon
GTTTCGTCTCTGGGCAGCGGTAATGATCACGTGATGGACGCCGTGTCTCAGTGTGAACAGTACGCTAAAGAGCAGGGCGCACAGGAGCGAAACGCCCCCTGGAGACTCTTCTTCAGGAAGGAGATCTTCACGCCGTGGCACGACCCAGCTGAGGACGCTGTCGCCACAAACCTCATTTACCAGCAGATCGTACGCGGCGTCAAGTTTGGTGAATACCGCTGTGATCGG
Seq A exon
GTATGAACTGATACTAATAGCCTGTGGTGCTTATAAAGGGCTCAAAATTAGCACTTTTCTCCATGCATAATTTAAACAGCCACAGCCATATCACCCTGCAGCCCAACACTGGTTACTCACTGAAGCTAATCAGGGCTGAGCCTGGTCAGTACTTGGATGGGAGACCACTAGGGAACACTAGGTTGCTGTTGGTAGTAGTGTTAGTGAGGCCAGCAGGGGGCGCTCAACCTGTGGTCTGTGTGAGTCCTAATGCCCCAGTAAAAGTGAAGGGGACACTACACTGTCAGTGGGCGCCGTCTTTCGGATGAGACGTTAAACCGAGGTCCTGACTCTCTTTGGTCAGTAAAAATCCCATGGCACTTCTCGTGAAAGAGCAGGGGTGTAACCCCGGTGTCCTGGCCAAAGTCCCTCTATCGGCCCTTACGATCATGGCCTCCCAATCATCCCCTTCCACCGAACTGGCTCTATCACTGTCTCTCCACTCCACCAATAGCTGGTGTGTGGTGAGCGCACTGGCGCCGTTGTCCTGTGGCAGCCGTCGCATCATCCAAGTGGATGCTGCACACTGGTGGTGGTGTGGAGAGACCCCCCTCATGATTGTGAAGCGCTTTGGGTGTATGGCCATGCACAATAAATGCGCTATATAAATATGCATTACATTACATTACATGTTCTCAACATGTTATTTTGCATTGACGAGATGAAGCGAAAATAAGATTATTTTGCTTTACTTTCAACATTGACACATTTTTTACTTGTTTTAAGAAAAATATGACTAATTTTTTACATTATTTCTGAAAACAACACAGTATTTTTTACTTACTGATTTAAGATTGTTTAGATATTCTGACTAAAAACAAGAAAAAAACTATAAAATAGCAGTTTTGGACACCAATCAATCTGGTTTCAAAAGTAGACACAACACTGAGACTGCACTAAGAAACGGTTGTCTGTGTTTTTGGACCGCTTAACTTTTCTATTTGACATGGCATCACACGGTGTGTCATTCAGAAACATGTAACGATGTCAGGATATCGATATGTCTGACAGATATTTTTTTTAGATGATGCACTTGTCACATTCGACTAAAGTTTTGTTCAGCAAACACAGACAATGCAGAAATCACATTAGGCAGATTCATTTGGTCACCTTCAGGGAGACCAGTACATAAGCAAAGTGCTATTTTGGGTCTTTTGAAACGCCATACCAAAAGTTTTCACTCACCCGCTGTATGTCTAATGTGGGGATATTATCTTTCTCTTGTTTGTTTTTTTACATTGATTTTTCCTTCTCTATCTATCCGTCTTGTGTGTTTTCCGGGTGAAG
Seq C2 exon
GAGGATCTGGCGGAGCTGGCGTCGCAGCAGTATTATGTAGATTACGGCTCTGAGATCCTCGTTGAGAGACTGTTGAGTCTGATTCCCTCCTACATCCCGGACAGAGAGATCAGCTCGGCCAAGACTGTGGAGAGATGGGCTCAGTTCATCATGGCTGCGCACAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099871:ENSDART00000166980:29
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF093795=FERM_N=PD(47.3=56.6),PF0037313=FERM_M=PU(29.0=38.2)
A:
NA
C2:
PF0037313=FERM_M=FE(55.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGCGGTAATGATCACGTGA
R:
GAGCCCATCTCTCCACAGTCT
Band lengths:
357-1683
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]