DreINT0101047 @ danRer10
Intron Retention
Gene
ENSDARG00000099871 | myo7aa
Description
myosin VIIAa [Source:ZFIN;Acc:ZDB-GENE-020709-1]
Coordinates
chr18:3166587-3168140:+
Coord C1 exon
chr18:3166587-3166744
Coord A exon
chr18:3166745-3167986
Coord C2 exon
chr18:3167987-3168140
Length
1242 bp
Sequences
Splice sites
5' ss Seq
TCGATATCC
5' ss Score
-5.09
3' ss Seq
CTTGACCGTGTGTCCTGCACCAG
3' ss Score
0.24
Exon sequences
Seq C1 exon
GCCTCCTCCTAAACACACTCTGAGTCGAGTGATGATCACCAAGAACCGAGGGAAGGATAAACTGTGGTGCTGCACTCGAGAGCCCATCAAACAGGCGCTGCTGAAGAAGTGTGGGGGACACGAGGAGCTCTCACAGGAGGCTTGCATGGCTTTCATCG
Seq A exon
ATATCCTTTCTGCACAATAGTTTCATATGTTTGCTTTGGTTTTTACTTAACCTAGAACATACAAACTTCATCATCATCATCATCATCATCATCATTTTCATCCGCTTATCTGGGGCCGGGTCGCGGGGGCAGCAGTCTTAGTAGAGAACCCCAGACTTCCCTCTCCCCAGACACTTACCTGGGTTTCCTCTGGGTGCTCCGGTTTCCCCCACAGTCCAAAGACATGCGGTACAGGTGAATTGGGTAGGCTAAATTGTCCGTGGTGTATGTGTGTGGATGTTTCCCAGAGATGGGTTGCGGCTGGAAGGGCATTCGCTGCGTAAAAACTTGCTGGATAAGTTGGCGGTTCATTCTGCTGTGGCGTACTTGGATTAATAAGGGACTAAGCTGACAAAAAAATGAATGAATGAATGAATTTATTATATAATTTTATTATATTTATAAATATTTATTTTTCTTAATTTTAAGCAGAAATGGGACGTGTTTGCATACAATAGGCTATTTTATGACCAATATTTATATTATATCATTCATTCATTCATTTTCTTGTCGGCTTAGTCCCTTTATTAATCCGGGGTCGCCACAGTGGAATCAACCGCCAACTTATTCACCACATTTTTACGCAGTGGATGCCCTTCCAGCCGCAACCCATCTCTGGGAAACCTCCACACACATTCACACACACACACACTATGGACAATTTAGCCCACCCAAGTCTTTGGACTGTGGGGGAAACCGGAGGAAACCCGGAGGAAACCCACGCGAACACAGGGAGAACATGCAAACTCCACACAGAAACGCCAACTGAGCCGAGGATCGAACCAGCAACCCAGCGACCTTCTAGCTATGTGGCGACAGCACTACCTACTGCGCCACTGCTTCGCCCTATATTATATTATATCATATAATAAATAAATATTTGATTTTTAAAAATATATATTTTTTAATAATAATCATTTTATATTTTTTCTTCATGTCTGGCTGACCTAGACCAGAAAATACTTTATGAAAAGCATACTTTAGTATATTTTTCAAATTAATTTTATTTGTATGTTAAATGATGCTTGCTTCTTCCACTTAATGCAACAAACACTTTCATATTTGTATTTATTTATATTAATTATGGGCTTAAATTACCTGGTCTCCCAAAAAGTTTTTTTTTTCCTTCAAATTAAGCTCTAAAATATCCTTTAATTTGCAGTGTATTGTGTGTTTGATCCTCCTTGACCGTGTGTCCTGCAC
Seq C2 exon
CAGTAATGAAGTATATGGGTGATTATCCGTCTAAACGCACACGCTCAGTGAACGAGCTCACGGATCAGATCTTTGAAGGAGCGCTGAAGGCTGAACCACTCAAAGACGAGATTTACTGTCAGATCCTTAAACAGCTGACAGAAAACCACATCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099871:ENSDART00000166980:36
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.053 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0078412=MyTH4=PU(34.6=71.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCTCCTCCTAAACACACTCTG
R:
TGATGTGGTTTTCTGTCAGCTGT
Band lengths:
311-1553
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]