DreINT0101061 @ danRer10
Intron Retention
Gene
ENSDARG00000099871 | myo7aa
Description
myosin VIIAa [Source:ZFIN;Acc:ZDB-GENE-020709-1]
Coordinates
chr18:3120864-3122785:+
Coord C1 exon
chr18:3120864-3120977
Coord A exon
chr18:3120978-3122631
Coord C2 exon
chr18:3122632-3122785
Length
1654 bp
Sequences
Splice sites
5' ss Seq
ATAGTGAGT
5' ss Score
6.97
3' ss Seq
TCTTCTCTGTGTAAACTCAGGGG
3' ss Score
5.15
Exon sequences
Seq C1 exon
GCCCGTGATGAGAGAAACTACCACATTTTCTACTGTATGCTGAAGGGAATGACTCCAGACCAGAAGAAGCAGCTGGGTTTGAGTAAAGCCACAGACTACACTTACCTCACTATA
Seq A exon
GTGAGTGATGATCAACACTGGCGCACGTTGAATATATGTGCTTCAGCCTACACAAAAATGTACTTCAACGCAGACTGACTGCAGTTTGTACTTTAAAATGAACACTAGGGGGCAGTATGACGCAAATAACTTTTGTTTTTTTCACACTGATGATGTTTACAGACACATGTTATTGATGAATAACGTATTATTTTAGTGCAGTTCTTTGCACAGCACAACTAAATACCACATAACAATGTAACAGTGTCTATGATTAGAAAGCGAATACGTTTGCCGCACATATCTATCTTCATACTCCTATATGCGTGGTCAGTTTGCTCAGTATTTTCCACGGTGTTGCTGTGCTTGTGCTGCAGAGCGCTCAGGTTTGAGTCAGGTGAAGCACACTTCCACATTGAGATGAAATCAATGTAAAATTTAGGCAAAACTACGTTGTCGCAGTTAAACTTTTTTTTATTTTTGCTTTTGAAAACACTATCGGTTGGGTTTAGGAAAGGGTTTGGGTAAGTCGTTCCCTATAGGTGATCTGAACTTAGATGTTTAAAAGAAGGACGTGTATCTGAAACAAACAAAACATATCTTAGGTAACGTATTTCAGATTCACAGATATGTAGATAAGTCCCTCTAGTGAATTTGTAATCTGAAACATGCAAAAACTGTACCCTAAATAACATATTTGAGATTCACAAAAATGTAGACAGCACCTTTTAGTAGATTTGTCATCTGAAACGTACATCAAAATGTACACTGAGTAACCTACTTCATATTTGGAAAAATGTTGACTGAGCCCTCTAGTCGATTTGTCATCGGAAATGTCCAACAAAACTTATCCTAAGTAACGTACTTCACATTTGGAAAAATATAAACAGCACCCACTAGTGGATTTGTCATCTGAAATGTCCAACAAAACTTACCCTAAGTAACCTATTGCATCTTCACAAATATGTCAGTGGCGCCCTCTAGTGGATTTGTCATGTAAAACATGCATAACTGTATCCTCAATAACATATTTGAGATTCACAAAAATGTAGACAGCACCTTTTAGTGGATTTGTCATCTGAAACGTACATCAAAACGTACACTAATTAACCTATTGCAGCTTTACAAATATGTAAACGGCGCCCTCTAGTGGATTTGTCATCTGAAACATACAGCAAAACTATCATAAAGCAGCAAACTTTACATTTGCATCAGTGAAGTTTCACAAACTAATTTCGAGAGAAGCACGTGATATGATTGTCTACTGCTGGCCAATCATCCGTAATCGGTAATAATCCAATCAGAATGATCCTAGCTTAATAAAAATTGATAACTTTCTCCTTATTGCTTCGTTTTGGAAGAATCTCCCCTTTCTCCTCCTTTTTCTCCCCTTCTAAAAGGGGAGCGATTGAGACCTACCTGACCTCGGATCCCCTGATATGCTTCTAGACCGGGCGGGAGCCCTAGGCTCAAATATCTCCGAGCTCAGGGTTCTCTCCCGGGACAGCATGCCAAACCTGCTATGAATGCCAAGCATATCTAAGTGGGAACTCTTGAAATGTCGGCTGAGGTGCATATTCCCAGTGAGCCTGGGTTAAATGATCTCCCCGATGATAGTCCAGATATGATCAGATGTGTCCTGCAGGTCTCTAATGTCTTCTCTGTGTAAACTCAG
Seq C2 exon
GGGAACTGCACTGTTTGTGACGGTCGAGATGATCAGAAGGAATACTCTAACATCAGATCAGCCATGAAGGTGCTCATGTTCACAGATAAGGAGAACTGGGAGATCTCCAAGCTGCTGGCTGCTATTCTGCACATGGGCAACCTCCGCTACGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099871:ENSDART00000166980:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.6=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(7.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCCGTGATGAGAGAAACTACC
R:
GTAGCGGAGGTTGCCCATG
Band lengths:
264-1918
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]