DreINT0101093 @ danRer10
Intron Retention
Gene
ENSDARG00000044632 | myo7ab
Description
myosin VIIAb [Source:ZFIN;Acc:ZDB-GENE-091110-4]
Coordinates
chr21:22261478-22263759:+
Coord C1 exon
chr21:22261478-22261641
Coord A exon
chr21:22261642-22263603
Coord C2 exon
chr21:22263604-22263759
Length
1962 bp
Sequences
Splice sites
5' ss Seq
GAAGTAGGT
5' ss Score
6.22
3' ss Seq
GTGTGTGTTTTTGTGTTCAGATA
3' ss Score
10.15
Exon sequences
Seq C1 exon
TTTGTTGCACCCATTCTGAAGTACATGGGCGATTACCCGTCAAAGAGGAGCCGCTCGGTCAATGAGCTCACTGACCAGATCTTTGAAGGAGCTTTAAAAGCTGAACCTTTAAAAGATGAGATCTACTGTCAGATCATCAAGCAGCTCACTGAGAATCATGTGAA
Seq A exon
GTAGGTTTTTACACTTTATATTTGTTGGGTTTCATTCACTGAGTTTACTTTTACTGCCTCTGAGAGTTTGGGGAATATTTAGCTTTTTTCTGTGCATAGTTGAGGGATGTCAGAATCATTTTATTGTTATAATTATTGTTAGTAGTAGTAGTAGTAGTTACAACAAAGGGATAATTCATTTAGGACTCAAAAATTACTTGTTTCAAACATGGGGTGCATTTCCCAAACAACGACATAACTCGCGGCTGAACTGTCATAGTACGATGCATTGTTTAGGAAAAGAACGATGTAGTGACGAGTGTTTTCCAATACCGTACTTTCTCTGTCGCAGATCCATCGTTTGAACCACAGTAGTTATAACATAAAATGTCCATAATGATGCTCTAAATGGGGTGGAGTAACAACTTCTTTAGAGAAAAGCCCAATAATATTTTGTGGAAGTTATATTGTTTTACACGCACATGCATATAAAAAAAATCTAATATTAGTTTTGTTAAAAACATGCACGCGTTTCCCATGTTAATTAAAATATATGTAAATGACACATATAGAACCTATGTTTCCTTTACAAATATTATTGAGAATATTCCATATAAAATAACTTAGCCAACATGTATACTAAACTTATATGTAAATCATGTAAATAGATAATAGTTCTAGGCCTAATTTACAGTAGGCTATTTATTAGGAAATGAAGAAAAAAATCCTACTTAAAAAATAATAATAAAGCAAAAAATACACAATGTACTACTTAATAATAACAACAACATTAATAACAATAATAATAATAATAATTTTTATTATTATTAATGTTGTTGTTATTATTAAGTAGTACAATGTGTATTTTTTTTTTTTAAGTCTACTTTTACAAGTTGACACAAGCGAACTACTGCAGAAATGTTCTAACCAACAGGCCCATGTCATATACACCCCCATCCCCCCCCCCCCCCCCCTTCACTTTGCTAACCGGTCACTATCGTTTTCACTTTCGGGTTGAGGGCTCCATAATCGACTTTTGCCTAAAGTGGCAGTTCAGCTTGCTCCGGCCCTGCCTATAGGAGTGATGACTTTAATGCTTTTTTTATAATTTCAAGTTCAAATGTTAGAATGTTCTAAATGTATAGGGCATAGGGGGGATAACTGGGAATAGAACAATTATTACTGGGAATTTTGCTTCTAACTACAGCTCTAGAGGTGTAGTTGTATGCGTACAAGTTTGCGATGAAGTTTGTGAATGTTTGTTTTAACAATGGATTTGGGAAACATCAAATCAACAAACTATGTTTGTAACAACGGAACTTGCGACCTTCGTTGGCTAGTGATGGTTTTCGGAAATGCACCCCTATTTGACTTTCTTCATATAAAAAAAGCTATTTTAAAGAAAGATGAAAACCTGTATCCGATGACTTTCTTAGTATTTGTTTTTCCTACTATGGATGTTAAAAGTTTTCAACATTCTTAAAAGTATCTTCTTTTGTGAAACTCAAAGGTTTATAACCTCGAGAGTGAGTAAATAGTAAGTTTTAGTTTGGGGGTTAAATGTGTCTATAATAAAATATTACGTAATACTCCTTACATCAAAGTATTAAATCTTTTTTATATTTTATTTATCTTTATTATATTCTATTCTTTACAACAAAAAAAAACATTTATGAACATTAAAAATGTCTGATGTGGTTTTTATTGTTTGTTTGGGTTTTTTATGCACATGTGTGGTTTGGCTTTCAATTAGGCTTAAATATCTGTGAAACTTCATCAATAAATCATTAACTTGCATGCAAATGTTTGCTTTAAGCACAGTTATTAAATGAAAGCCATTACTGTAAAAGCAGTTGGTTTGGCTTGTAAATCTGTAACATTCTGTCCTCTAGTGGTCAGCCTGCAAAAAGCCAATGTCTAATGAATGGCCGTTTGAATGGTCTGACCGTGGTGATGTATGTGTGTGTGTGTTTTTGTGTTCAG
Seq C2 exon
ATACAGTGAGGAGAAGGGCTGGGAGCTGCTCTGGCTCTGCACTGGACTCTTTCCTCCCAGTAACATGTTGCTCCCTCACGTCCAGAAGTTCCTGCAGTCCAAGAAACATCATCCGGTTGCTCCAGACTGCATGCAGAGACTGCAGAAAGCCCTGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044632:ENSDART00000148019:36
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0078412=MyTH4=PU(34.6=66.1)
A:
NA
C2:
PF0078412=MyTH4=FE(48.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTGTTGCACCCATTCTGAAGT
R:
CAGGGCTTTCTGCAGTCTCTG
Band lengths:
318-2280
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]