DreINT0124798 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35862369-35864090:+
Coord C1 exon
chr2:35862369-35862571
Coord A exon
chr2:35862572-35864002
Coord C2 exon
chr2:35864003-35864090
Length
1431 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
3' ss Seq
GTGTCATTTGTGATTCATAGGCT
3' ss Score
7.08
Exon sequences
Seq C1 exon
TATGAACAGGAAATCTCGAAGCTAAAGGAGCGTCTGCGGACGTCTAGTCGGCGGCTGGAGGAGTATGAGAGACGGTTACTGGCTCAGGAGCAGCAGATGCAGAAGCTGCTCTTAGAATACAAGACTCGACTGGAGGACAGTGAGGACCGTCTGCGAAGACAACAGGAGGAAAAAGACAGCCAGATGAAGAGTATTATCTGCAG
Seq A exon
GTGAGTGAAGATTGTTGTTCTGAATACATTAATGAAATTAATACATTACTGATACAAATAATACATTAATTAAACAAATAAACTTTTAAATACAAATATTTATTTTAATATTGCAGTTATTTGCTCAAGTGTTAAAGCCAAATATAAAAAAAATGTTGCCATAATAATCTGGATTCCGTTAAAGGAAGTTTAAATGTGGAAATGTCTATTCCAGACATTTAAGTCATGTAATTTGTTAGTCACATAAGTCAATTGCTGTCAAGAGACTGTAATCGTTTACTAAGGCTAAAATTTCAGAAAAATATACATTTTAAGAGGTTTGACCACTTATGCCAAGTTCAGACTGCATGATTTTAGCCCTGATTTTGCCGCCGACAACAGGTTTTGATAAATCTCAGACAAATGCCTGAAATCGCAGGCAAATCGGTGCTCGTTTACGTGAGTAACAATCAAAGACGCGATCTAAGAAAGTCGCAGATTATTCAAATCATGCTGTGTGAAATGAGTTCTGACTGAAAATAACATCTGTGATTACCTACAGCCAATGAGAGAGCAGCATTCACTAGTGAAGGTATCTGCAGGCCAGCTGGAGGTTAGGGGAGAAGTTAAAAAGCGACACTTTCAGTTTATTTGGACCCAAGAAATGGAGAAAAAAACTAGTGTAAATTTGACAGGAGCACTTGTGTCTGTTTGACGTGACATCTGAGCAATATCACAACCGGGTGAAAAAGAAAAAGTTAAGGAGAAATGGGTAATTCCCTTCAAAAGCCAGGAGAGCAAAATAAGTACATTTTCTACCCCACTAAAGGCTTCTTTCTCATTATGTAGTTAATAACAAAAGATATACTATGTGACCAAAGGGACGAAGTTGTCAGCGATTCTCTTATTGTAAAGTAAAAAAAAAATACCCCTTATATCCTTAAAATATAGCTCCCTTTATATATTAAGAATATAAAGGGTATTTTTTCTTTTGTTAATTTCTAAAGTTTGGAATCGTTTCAGATTTAAAAAATAAAAAAATTATCTGAAAACGACTTTGCTCTTAAAATGTCTAATTTACTTCAGTAAAATTGTTGTGTTTTTTTATATTTGGCAGCTACAATTTAATAAAGCAAGCAACTTTTTTTACCATATATTTTATAATATTTTTCTTCTGGTAAAAGTCGTATTTATTTTAGTTTGGCTGGAATAAAATCTGGTTAACCTACTTAAGTCTTTTAATTGCACTTTAAGCTGAACACTAGTATTTTGCAAGATAACTAGTAAAATGTTCTATACTGTCATCATGGCAAAGAAGGTTAAAACATCATTTGGAAAATATTTTTTAAAGAATTACAATTTTCAACAGAGAGCTGATAATTTTAACTTTAACCTATCTGTAATTGTAAAACCATGTGATATCTGACTAAATGTGTCATTTGTGATTCATAG
Seq C2 exon
GCTGATGGCCGTGGAGGAAGAGCTGAAGAGAGACCATGCAGAGATGCAGGCAGTCATAGATGCCAAACAGAAGATAATCGATGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.544 A=NA C2=0.222
Domain overlap (PFAM):
C1:
PF120043=DUF3498=FE(12.5=100)
A:
NA
C2:
PF120043=DUF3498=FE(5.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAACAGGAAATCTCGAAGCT
R:
GTGCATCGATTATCTTCTGTTTGGC
Band lengths:
287-1718
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]