MmuINT0132039 @ mm10
Intron Retention
Gene
ENSMUSG00000070565 | Rasal2
Description
RAS protein activator like 2 [Source:MGI Symbol;Acc:MGI:2443881]
Coordinates
chr1:157146190-157147832:-
Coord C1 exon
chr1:157147630-157147832
Coord A exon
chr1:157146278-157147629
Coord C2 exon
chr1:157146190-157146277
Length
1352 bp
Sequences
Splice sites
5' ss Seq
CAGGTTAGA
5' ss Score
5.94
3' ss Seq
TTCTTCCTACCACATTCTAGGCT
3' ss Score
7.02
Exon sequences
Seq C1 exon
TATGAACAAGAAATTACCAAGCTGAAGGAGCGCCTACGAGTGTCCAGCCGGCGGTTGGAGGAGTATGAGCGCCGGCTGCTGGTGCAGGAGCAGCAGATGCAGAAGCTGCTGCTGGAGTACAAGGCCCGGCTGGAGGATAGTGAGGAGCGGCTGCGCAGACAGCAGGAGGAGAAGGACAGCCAGATGAAAAGCATCATCAGCAG
Seq A exon
GTTAGAAGACCACCTGCTCTCTGATTAGCTCTAGTTAGGTACAAAGAAATGGGAAAATGGTCCAAATACTTGGGAAAAAGTTTTCATCCATGTTTATAAATATATTTTGTAGTACAGTACTCAGTACAAATGTAATTAATGACTGTGTCTCTCAGGCATCGGCCCAGATGAAATCTGGTATACTTTTATGAAGATAAAGTAGGACCTTTTTTTCTTTAACCATAACAATATTAGAGGAAAAAAGAGGGGGAAACTAGGAATTTACTTTGAAGTTTTAGATAAAGATTGGGTTTTCAAAATCTACAATACTAAGATTTAACTTGGTTTTCCCAAGACAAATCCAAAAACAAAGCAGTCTCACTGGATGTCTTTAGGGCCAGACCAAAAAGTGCCATAGATGCCAAAAAAGGAAAGAAGAAAAATGTCTAACTGTATTTGCAGATCACTCACTATGGCCAAGTACTTTTTTAATCACTTTGTAAATAGTACTTTGTTTTATCTTATCTGCTCTCTGAACCAGATATTATTATCACTTCTTTACAGATAGAAAAATTGTCAATACTTGCATGCCCAAGGTAGCATAGTCAGTGGAGACCTAGGATTGGAGCCAGGCATCAGGCTTCAGATCATAACCACTAGCTTTGATCATTCTACATACTGTGGGCAAAGAAAGAGAACATAAAAAGGGTTGCTGTCCGAGTAGCCTGGAAGTGACCTGGTACTAAGAGTGATGGGACTAGAACTGAAGAATTGTGCATAAATGGATTGAGATGTGAGGAGGAAACGAGATCTCATGGAATGCCCTGTAGGAAACATTACTGTCAAGGGAGAAGGGAGACAGGATGGTAACTACAGTATAACTCAAGAGGAAGAGTAGATGGTTTGCAGCAGGCATGCTGGTGCTTGCACATACTAACTCCAGCATTCAGGAGGTAGAGAGGAGTACCTGGAGTTTAAAGCCAGCATCAATTTCATAGCAAGCTTAAGGCTAGCCTGAACTACATGAGATACTCTCTCAATAATAAATAATGTAAAAAGTAGATAGCCTTTTTTTTGTTTTGTTGGTTTTGTTCATTTGCTCTTTTAAGATGCTTGCAAAAAGAGTAATCTAATAGGAAGATGATTACTAGAATATTCCTCTTAAGAAAATGTAATGTTATAGACTCAATCACAGATAAAAGACTCAATTAAGACGTCTTCTTTTAAGGGCAGATAGGAGAGGAGAAGAAAGAAAAGGTTATTACAATAAAAGAGGTCTTCTGTTGGGAAACAGATGTTTTAAGTTTCTCATTCTCTGTGTTGCCTAGCCCTGTCTTTGAAAATATCAATACCTTCTTCCTACCACATTCTAG
Seq C2 exon
GCTAATGGCTGTGGAAGAAGAATTAAAGAAAGATCATGCTGAGATGCAAGCAGTTATTGATGCAAAGCAGAAAATAATTGATGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000070565:ENSMUST00000078308:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.364 A=NA C2=0.192
Domain overlap (PFAM):
C1:
PF120043=DUF3498=FE(13.3=100)
A:
NA
C2:
PF120043=DUF3498=FE(5.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAACAAGAAATTACCAAGCTGAAGG
R:
GTGCATCAATTATTTTCTGCTTTGCA
Band lengths:
287-1639
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types