DreINT0124805 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35854422-35855176:+
Coord C1 exon
chr2:35854422-35854520
Coord A exon
chr2:35854521-35854621
Coord C2 exon
chr2:35854622-35855176
Length
101 bp
Sequences
Splice sites
5' ss Seq
AAGGTGACT
5' ss Score
6.64
3' ss Seq
TTAGTGTAATTCTCCTCCAGGAT
3' ss Score
7.8
Exon sequences
Seq C1 exon
GTGGCGTACTCAGGTGGAAGTAAGTGCTTCAGCTGTTCCTCTGCTGCTGAACGGGACAAATGGATGGAGAACCTCAAAAGAACTGTGCAACCTAATAAG
Seq A exon
GTGACTGTCATATCTTTTGTTTTTTAATATAATCAAAAGCATGCATGCTTATTTTATACTTAAAAGAGCTGGTATGATTTATTAGTGTAATTCTCCTCCAG
Seq C2 exon
GATAATTGCCGGCGGGCGGAAAACGTCCTCCGCCTGTGGATCATTGAGGCAAAAGACCTGCCACCAAAAAAGAAATATTTCTGTGAGCTGTGTCTGGATGACATGCTGTACGCTCGTACCACCAGCAAAACCCGCTCCGACTGCCTGTTCTGGGGGGAACACTTTGAGTTTTCAGGCCTCCCGTCCATGAAAAGCATCACCATACACATCTACCGGGATGTGGACAAGAAAAAGAAAAAGGACAAAAACAACTATGTTGGCCTGGTGAACATCCCTGTGCATGGGGTGATGGGTAGGCAGTTTGTGGAGAAGTGGTATCCAGTCAGCACTCCCACCACCAGCAAAGCCAAAGGTGGAGGCCCCTCCATCCGCATCAAATCCCGCTTTCAGACCATCTCAATTCTGCCCATGGAGCAGTACAAGGAGTTTGCGGAGTTTGTTACCAATAACTATACCATGTTGTGCTCTGTGTTGGAGCCGGTCATAAGCGTGAAGAACAAAGAGGAGATGGCCAGCGCTCTGGTGCACATTCTGCAGAGCACGGGACGGGCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.114 A=NA C2=0.011
Domain overlap (PFAM):
C1:
PF0016924=PH=PD(11.6=87.9)
A:
NA
C2:
PF0016825=C2=WD(100=43.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCTGAACGGGACAAATGGA
R:
ATGCTTTTCATGGACGGGAGG
Band lengths:
253-354
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]