DreINT0133536 @ danRer10
Intron Retention
Gene
ENSDARG00000099021 | setdb1b
Description
SET domain, bifurcated 1b [Source:ZFIN;Acc:ZDB-GENE-061013-224]
Coordinates
chr16:22096759-22098847:-
Coord C1 exon
chr16:22098678-22098847
Coord A exon
chr16:22096999-22098677
Coord C2 exon
chr16:22096759-22096998
Length
1679 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAA
5' ss Score
8.59
3' ss Seq
ATATTTTGTTTCTTTCTCAGGAA
3' ss Score
10.31
Exon sequences
Seq C1 exon
GATCTATGAATGTAATAAGCGGTGCCGCTGTAATATGCAGATGTGCACTAATCGTCTGGTTCAGCACGGTTTGCAGGTCAGACTGCAGCTCTTTAAGACCCAGAATAAAGGCTGGGGGATTCGGTGTCTTGATGACATCGCCAAGGGCTCCTTTGTTTGCATCTATGCAG
Seq A exon
GTAAAACACTAAGAAAAAGCCTTAGACTGGGGGTGTACAATGTTTACGATAATAACATAATATATTAAAATCATATTTTTATATATATTTATAATGATGTGTGACTTAACATTGTTGCTCATTCTGCAAAAAAACAACAACAACAAAAACACATCTTGAGTATTCAAAGCATTCACTTAATGTTGTGTTTTGTTTCAGTGAAGATAGAATGACTGTTATGGAACATTGCATTCCTTGTTTATGATATTGTTAAGTAGGGCTGCACGATTTGGTGGAAAAAAATTAATTGCAATTTATCTGATTTGGGATTTTTGTGATTTTTAAAATGCTATATACTTTAATTTCATAAAAGAACTGCAGGTTTGATTTGGTGGTTTTAACAGCACCAAAGAAATACCAAGCATAATTACAAACTACACTACACTACACTATGCTACTGTTTATGTAAAACCATTTTTCCCAATAAAGTTGTCAGGACAAATGAAAACAATGACTAAAGTATTTTAAATTTAATTAAATTATATATAAAGTCTTATATATAAATATATAAAACCTTTTACATTTTGGTTGTTACATTTTTCTTATTGATTTTATGACATTCACGATGCATTGATGATATTTTCCATAAACATTTTTAGATTTTGCTTCATAGCACAAGAATTCTTGTATTAAAACGAATAATTTATAATTATATTTATATATTATTTGTAATACAATTTTGTCCTTTAATACAAGCAGTCAGATAAATGAAGTGTACCTTTAATTTGACCTGCTCAAAGAAAACTCTCTTTTTGAATGCATCAAACAAAACTTGAGTAAATACACAGAACAATATAAGCATTTATAGAAAATAAACTAATGTAAATATTATTGTGTGTGTGGAATAACAAGAATCAATGTAACAGCCTAAAGAGGGGACGAAACGTTACCTTCCGCAAGCAGGTCAAAGGTCCACACATATGCAGAGTAGAGATGGAGTTCACAGCTTTCATTTAATCTCCATGGCAGTGAAATAGGGGCTTGTGGGTTCTGCTGTAACAGTTGTCTGCTATTTAAGTAATGTTAGTTGGATCGTTTTAATTTTGCGGGCTCGTCTCCCTCACCATAGTAATAATTGAAATAATTGCAGCTTTTCAAATTACTATTTTAGTTTAAAATCGATTAATCGTGCAGGCCTATCGTTAAGTACAATCACACCAACAAATACATTTTCCTGAATTCCAGAGTATTGAGTTAGATTTCATATACATTATTGTCTGTTTTTTTTTTTCTTTTTTTATTTAAGAGTTAAGCTGCAAAAATCTGCCATAAAACCTTTCACAAAAAGTGCCAGCAGAAATCTTTTGTGGCCTATGATTTTTTTTAAGCCTCTTATATTCATGTTCAGTTATTTCATGTTTAAAGTGATGAAAATAATTGCTGAAAATTCTTTGCTATATAAGTAAAATTACTGAATTGAGCCTGAGAAAAATGCTAAACGATACTGCAGTATAAAATGTTTTTAAAAAGTATTACACATCCCAACTTCAGTTCAATAATAGGAGAATGACTCCAGTGTTTAGTCTTGTATTAGATCTCCCTTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTATATATATTTTGTTTCTTTCTCAG
Seq C2 exon
GAAAGATCCTGACAGATGACTTTGCCGATAAAGAAGGTTTGGAAATGGGCGATGAGTATTTTGCCAATCTAGACCACATTGAGAGCGTGGAGAACTTTAAGGAAGGTTACGAGAGCGAAGCCCACTGCTCGGACAGCGAAGGCAGCGGTGTGGACATGAGCAGAGTTAAACTACCTGCTTCCTCCCGACACGGTAAATCCAACAAGATGGAGGAGCGTAACAGCAGCACAACTGGCAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099021:ENSDART00000165824:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.436
Domain overlap (PFAM):
C1:
PF0503311=Pre-SET=PD(15.7=31.0),PF0085623=SET=PU(29.0=34.5)
A:
NA
C2:
PF0085623=SET=FE(29.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACGGTTTGCAGGTCAGACTG
R:
TTGCCAGTTGTGCTGCTGTTA
Band lengths:
344-2023
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]