DreINT0150009 @ danRer10
Intron Retention
Gene
ENSDARG00000035468 | slc25a25b
Description
solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25b [Source:ZFIN;Acc:ZDB-GENE-060526-340]
Coordinates
chr5:31195459-31196930:+
Coord C1 exon
chr5:31195459-31195546
Coord A exon
chr5:31195547-31196818
Coord C2 exon
chr5:31196819-31196930
Length
1272 bp
Sequences
Splice sites
5' ss Seq
GAGGTGTTT
5' ss Score
1.54
3' ss Seq
TGTTGTTTTTATCTGAACAGTAT
3' ss Score
7.89
Exon sequences
Seq C1 exon
GTCACATTGATTCGCAGGAGATCATGCAGTCACTACGAGACCTTGGGGTTCATATATCTGAGGAACAGGCTGAGAAGATTCTCAAGAG
Seq A exon
GTGTTTATTTTTTTTTTTATTTTTTTTTTTTTTTACTGATTTGTATCATCAGTCCATCTTTCTTTTTAAAAGCTGACTGATCTATGTACAGTAGCATCTAGGTGACGGAAAGAACACAGCATACAGTTAACACAGCATATCTCACAAATTTCTACACTTCATTGTTTTTTGACATTCTTAAGCTTCTTAAATGTCTTCTCCAACAGTACGAAGCAAACTAATTGAGGCACAAACGTTCTATTTCTTTTCTGTCTGTGTGACCGTGTACTGTGTGCGTGTAAAAGTCTTTCGCTTTCTGTATGTGTTTATCACTCTCTCCTTCTTGTGATCCACCTCTACCCTCTCTCTCTGCTCATCTTATCAGAATAAGGAGGGGTCATATTTGTGCCCCTATAATGTAGTAAGTAGAGGTCTTCTCCCTGCACGTTTGCCTTGCTCTGCATTTGCATTTCATCCGCTGTGTGTGTCATCTGTGCTGCTTTGTTTTGGGTTTTTTTTTTCCTCTCTCAGTGGCTGTTTTGTCCCATGTGAGTGTTTTATTTTGTTTTTTTAAAGGAGCTAATGTGGATGTAATTTAGGGTGTTTTACAGGGCATTTCCACAGAGGCACTCTTTCTTAAACTTATTTAAAGAAGATAAATACGTGAAGGTCAAAATGGTGGTGAAGCTCTGAATTCAGAGACTTTTTTTGCATGCAAATATGGAAAAAATTAAGAAACTACTTGAAAATTCTAATCATTTTGAGGATTTACTGAATTTATTAGGTATGTGCTTGAGTAAAAATATTGTTTTATTCTGTATTAAGCCCTGATAATTTTTATTCTTTTGAGAGAAATGACAGTTGTTTGAACTCTTAGTCAAAATATTTTCATTTGTTGTAAATTATCAGGGCCACATATTGCTTCATACACTTACTGGAAGTCAAAATATTCCCTTTGAGTTAAATAAAGACTATGTTTCAGAACATGCTTGACCAGTTATCATTTTAGCCACTAAATGACAACATTTTTATGTTATATTTGAAAGAAATGTCAACAATAGTTTGCAGAACAAAGCACTAATTATATATTACTCAAACTGTGGACTCTTCTTTTTTTTCCCCATTTAATGTGGATGTTATTTTTATTTTATTTTTGCAGCTTGATTGTACATGATGAATTTTGCTAGAATAAAGCTGGTCTCATTTAGATTAGCTGCTGCTATCCAGAGCCACAGTTAGTATATAAACATTATTTAATTTTTTGCTCTCTGCTTGTTGTTTTTATCTGAACAG
Seq C2 exon
TATGGATAAAAATGGTACGATGACGATTGACTGGAACGAGTGGAGAGACTATCACCTCCTTCACCCCGCCGAAAACATTCCCGAAATCATCCTCTACTGGAAACACTCTACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035468:ENSDART00000098163:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF134991=EF-hand_7=FE(43.3=100),PF118483=DUF3368=FE(76.3=100)
A:
NA
C2:
PF134991=EF-hand_7=PD(25.4=44.7),PF118483=DUF3368=PD(5.3=5.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCACATTGATTCGCAGGAGA
R:
CGTAGAGTGTTTCCAGTAGAGGA
Band lengths:
200-1472
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]