DreINT0154669 @ danRer10
Intron Retention
Gene
ENSDARG00000056666 | sp8b
Description
sp8 transcription factor b [Source:ZFIN;Acc:ZDB-GENE-030131-3654]
Coordinates
chr16:19730820-19732953:+
Coord C1 exon
chr16:19730820-19730885
Coord A exon
chr16:19730886-19731254
Coord C2 exon
chr16:19731255-19732953
Length
369 bp
Sequences
Splice sites
5' ss Seq
GGGGTGAGT
5' ss Score
7.93
3' ss Seq
TGTTGGTGTTGTTGTTTCAGGAG
3' ss Score
8.92
Exon sequences
Seq C1 exon
TGATTCATAAATATAAAAAGATCCTCTGAGGAGGGCACTTTTGTGATGGCAACTTCACTTCTAGGG
Seq A exon
GTGAGTCTAAGGAGTTTCGTGCTGGTTTAATTAGTTTCCTTTATTGTCCTGCAGTGGGGATTAAAATTTCTTTGGTCAGCCGGGGAGAGAGTCACTTGATTATGTTTTAAGCGAGAGAAAGGAGCAGGAGTTGCATTTCTCAACTACGACATTGTATGTATAACACATATTGCTATTTCTATGCTGCGTTTTCGCTTTTGTGTTGCGTGTGCAATTGGCTGTTTCAGTTGACTGTTCTTCGCTACCATTCTTCAGGTGTGTTGGTTAAAAGAGTAAACCGTTAAAACTGTTCTTTTAATTCCCCCAGTTCCCCGAAACAAAGTCTGAACTTACCTTCTAACTGCTTTTTTGTTGGTGTTGTTGTTTCAG
Seq C2 exon
GAGGAGCCGAGATTGGGGTCGACTCCTTTAGCCATGTTGGCTGCGACGTGTAACAAAATCGGGAGCCCGAGTCCTTCTCCTTCCTCGATTTCGGATAATTCATCTTCGTTCGGAAAAGGATTCCACCCGTGGAAACGAGCGACAGCCAGCAGCTGTAGTTTAGGATCCAGTTTATCCTCTTTCACTCGGAATGGTGGTGGTTTAAGCGACTCTTTTGGCACGAACACTTCTACGGGATCAAGTGCCTTTTCCTTAACTTCTGGTACTTCGTCCAACTCCCACTTCGGAAACGACTACTCCGTTTTCCAGACTTCTGTGTCAAATAACTCCCAAGAGCCAAGCCACCAACCCATGTTTATTTCAAAAGTGCACACATCCGTGGACAGTTTGCAGAGCATTTACCCGAGAATGAGCGTGGCGCATCCCTACGAGTCGTGGTTCAAGTCCTCACACCCTGGCATACCCACGGGGGATGTGGGCACCACGGGCGCGTCCGCGTGGTGGGACGTGGGAGCCGGTTGGATTGATGTGCAGAACCCGAATGGAGCTGCGCTCCAGACGTCCCTACACTCCGGTGGGCTGCAAACTTCTTTACACTCTCCTCTGGGCGGATACAACTCAGACTACTCGAGTTTAAGCCACTCTGCCTTCAGTACCAGCGCGTCCCCGCATCTCCTCACCACCGGGCAGCACCTCATGGACGGATTCAAACCGGTTCTCTCGTCTTACCCGGACTCCAGTCCTTCTCCTCTGGGTGGAGCGGGTGGTTCCATGTTGACAGGGGGTCCGACGGCCTCTTTGGCGGGGTCCCCGCGGTCTTCCGCGCGGCGCTACTCGGGCAGAGCGACGTGCGACTGTCCGAACTGTCAAGAAGCTGAGCGGCTGGGTCCGGCCGGCGCAAGCCTCCGCAGAAAGGGGCTGCACAGCTGTCACATCCCTGGCTGTGGGAAAGTGTACGGCAAAACGTCTCACCTCAAGGCTCATTTGAGGTGGCACACCGGGGAAAGGCCGTTTGTGTGTAACTGGTTATTCTGTGGAAAGAGGTTCACGCGCTCCGACGAGCTCCAGCGACATTTACGCACGCACACTGGCGAGAAGCGCTTCGCTTGTCCCGTGTGCAACAAGCGCTTCATGCGGAGTGACCACCTCAGTAAACATGTCAAAACGCACAGTGCTGGGGGCTCGTCAGGGTCGGGGTCTGGGGCGAGTGGAGGGAAACGAGGGAGTGACACCGACAGCGAGCACAGTGTGCCCGGTAGCCCCTCATGCCATTCGCCTGACCTGTTGCAAGCCCCCGAGTCTGTCCCGACCACTGGAATGAACGGCCGTGCAAGCTCCCTCGATTAATCCCGCTCTAAATGCCAAGCTCGAATGGACTAGTAACGTATTTGACGCCTCAGAAAATGTACTGTTCGCTCGGAAACACTTTCTAAAAACTTTCAAGTTAACAAAAAATCTAAAATGTTTTGCAAGTTTCCGATTAATGCACATTCAAATATGAATAACCTACATGAAACAACATGAACCAAAGTTTCATTTCTTTTTAACTAGTTGAATGTACATTAATGAATGTTAATTAGGCCTATATTTTTTTTGTTAACAGTTAAAGTTATTATAAAGTGTCACCTGAGAAGGAACCGTGTGGCCATTTCACGTGGATGTTGTAAAGTGATTCAGTTTACAATTAAAATGTAATTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000056666:ENSDART00000079201:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.364
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF134651=zf-H2C2_2=WD(100=6.2),PF134651=zf-H2C2_2=WD(100=5.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCTGAGGAGGGCACTTTTGT
R:
CGGAGTAGTCGTTTCCGAAGTG
Band lengths:
344-713
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]