GgaEX0002970 @ galGal4
Exon Skipping
Gene
ENSGALG00000002364 | SEC16A
Description
SEC16 homolog A, endoplasmic reticulum export factor [Source:HGNC Symbol;Acc:HGNC:29006]
Coordinates
chr17:7870360-7872142:+
Coord C1 exon
chr17:7870360-7870434
Coord A exon
chr17:7871154-7871213
Coord C2 exon
chr17:7872068-7872142
Length
60 bp
Sequences
Splice sites
3' ss Seq
GTGTTCTTGCTGTTTTGTAGCTT
3' ss Score
8.88
5' ss Seq
CAGGTACAT
5' ss Score
7.79
Exon sequences
Seq C1 exon
TATTTAAACCCTGCAATTGTTCCACCTGGATCTGGGCTTCCTGTCTCCAGCCCTGACAGCTTCCAACCAGGCGAG
Seq A exon
CTTTCGCGCTCTAGTTCAATGAGTTCATTATCACGTGAAGTAAGCCAGCATTTTAATCAG
Seq C2 exon
CCTGCCCCTGTGCCGCCTTCAGGGGAACCGTCAGCAGGAACAGTACAGTTCTACAACCCTTCTCAATTTGCACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000002364_MULTIEX2-2/2=1-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCCTGCAATTGTTCCACCTG
R:
TGTGCAAATTGAGAAGGGTTGT
Band lengths:
142-202
Functional annotations
There are 1 annotated functions for this event
PMID: 27492621
Activation-induced alternative splicing of Sec16a (inclusion/exclusion of HsaEX0056828 and/or HsaEX0056829) controls adaptation of COPII transport to increased secretory cargo upon T-cell activation. Using splice-site blocking morpholinos and CRISPR/Cas9-mediated genome engineering, the authors show that the number of ER exit sites, COPII dynamics and transport efficiency depend on Sec16 alternative splicing. As the mechanistic basis, the authors suggest the C-terminal Sec16 domain to be a splicing-controlled protein interaction platform, with individual isoforms showing differential abilities to recruit COPII components. These work connects the COPII pathway with alternative splicing, adding a new regulatory layer to protein secretion and its adaptation to changing cellular environments.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]