GgaEX0010414 @ galGal3
Exon Skipping
Gene
ENSGALG00000007330 | Q5ZJ21_CHICK
Description
NA
Coordinates
chr5:18133879-18134743:-
Coord C1 exon
chr5:18134590-18134743
Coord A exon
chr5:18134181-18134198
Coord C2 exon
chr5:18133879-18133947
Length
18 bp
Sequences
Splice sites
3' ss Seq
CCTCTCCGCTTCGCCAATAGACG
3' ss Score
6.56
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
Exon sequences
Seq C1 exon
CTGGTGGAGAATTGTGTGTGCCTGCTGAGAAACTTGTGTTACCAAGTCCATCGTGAGATCCCCCATGCTGAGCGCTACCAGGAGACTCCTCTGGCCACCACCAACAACGCTGGGCCCCACGCTGCAAGCTGCTTCGGTGCCAAGAAGGGCAAAG
Seq A exon
ACGAATGGTTCTCCAGAG
Seq C2 exon
GTAAAAAGCTCCCTGAAGACCCTGGTGCAGATACAGTGGATTTTCCTAAAAGAACAACTCCAGCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000007330_MULTIEX2-1/2=C1-2
Average complexity
S
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.197 A=0.429 C2=0.573
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0051418=Arm=PU(2.5=4.2)
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAGCGCTACCAGGAGACTC
R:
CCAGGGTCTTCAGGGAGCTTT
Band lengths:
112-130
Functional annotations
There are 1 annotated functions for this event
PMID: 32423416
The authors hypothesized that AltTEM regulation was responsible for a protein localization change in the Ctnnd1 protein (aka p120). Validation experiments using Western blot analysis of p120 in nuclear/cytoplasmic fractions for both cell types confirmed the increase of nuclear levels of the protein in OPCs in comparison to NPCs, where a cytoplasmic retention of p120 was observed. Higher inclusion, higher nuclear localization.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]