GgaEX0010414 @ galGal4
Exon Skipping
Gene
ENSGALG00000007330 | CTNND1
Description
Uncharacterized protein [Source:UniProtKB/TrEMBL;Acc:Q5ZJ21]
Coordinates
chr5:16249219-16250083:-
Coord C1 exon
chr5:16249930-16250083
Coord A exon
chr5:16249521-16249538
Coord C2 exon
chr5:16249219-16249287
Length
18 bp
Sequences
Splice sites
3' ss Seq
CCTCTCCGCTTCGCCAATAGACG
3' ss Score
6.56
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
Exon sequences
Seq C1 exon
CTGGTGGAGAATTGTGTGTGCCTGCTGAGAAACTTGTGTTACCAAGTCCATCGTGAGATCCCCCATGCTGAGCGCTACCAGGAGACTCCTCTGGCCACCACCAACAACGCTGGGCCCCACGCTGCAAGCTGCTTCGGTGCCAAGAAGGGCAAAG
Seq A exon
ACGAATGGTTCTCCAGAG
Seq C2 exon
GTAAAAAGCTCCCTGAAGACCCTGGTGCAGATACAGTGGATTTTCCTAAAAGAACAACTCCAGCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000007330_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.202 A=0.286 C2=0.521
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0051418=Arm=PU(2.5=4.2)
Main Inclusion Isoform:
ENSGALT00000011857fB1162
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAGCGCTACCAGGAGACTC
R:
CCAGGGTCTTCAGGGAGCTTT
Band lengths:
112-130
Functional annotations
There are 1 annotated functions for this event
PMID: 32423416
The authors hypothesized that AltTEM regulation was responsible for a protein localization change in the Ctnnd1 protein (aka p120). Validation experiments using Western blot analysis of p120 in nuclear/cytoplasmic fractions for both cell types confirmed the increase of nuclear levels of the protein in OPCs in comparison to NPCs, where a cytoplasmic retention of p120 was observed. Higher inclusion, higher nuclear localization.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]