MmuEX0013079 @ mm9
Exon Skipping
Gene
ENSMUSG00000034101 | Ctnnd1
Description
catenin (cadherin associated protein), delta 1 [Source:MGI Symbol;Acc:MGI:105100]
Coordinates
chr2:84452181-84453246:-
Coord C1 exon
chr2:84453093-84453246
Coord A exon
chr2:84452689-84452706
Coord C2 exon
chr2:84452181-84452249
Length
18 bp
Sequences
Splice sites
3' ss Seq
CCTTGGTGATGCACTGGAAGATG
3' ss Score
-0.57
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
Exon sequences
Seq C1 exon
CTTGTGGAGAATTGTGTTTGCCTCCTCCGGAACTTATCATACCAAGTTCATCGTGAAATCCCACAGGCAGAGCGTTACCAGGAGGCCCTTCCTACTGTGGCTAACAGTACTGGGCCACACGCTGCCAGTTGCTTTGGAGCCAAGAAGGGCAAAG
Seq A exon
ATGAGTGGTTCTCCAGAG
Seq C2 exon
GGAAAAAGCCTACAGAGGATCCAGCAAATGATACAGTGGATTTCCCTAAAAGAACTAGTCCTGCTCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034101_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.224 A=0.571 C2=0.708
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0051418=Arm=PU(2.5=4.2)
Other Inclusion Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCAGAGCGTTACCAGGAG
R:
TGCTGGATCCTCTGTAGGCTT
Band lengths:
116-134
Functional annotations
There are 1 annotated functions for this event
PMID: 32423416
The authors hypothesized that AltTEM regulation was responsible for a protein localization change in the Ctnnd1 protein (aka p120). Validation experiments using Western blot analysis of p120 in nuclear/cytoplasmic fractions for both cell types confirmed the increase of nuclear levels of the protein in OPCs in comparison to NPCs, where a cytoplasmic retention of p120 was observed. Higher inclusion, higher nuclear localization.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: