GgaEX0019786 @ galGal3
Exon Skipping
Gene
ENSGALG00000013124 | FHOD3
Description
NA
Coordinates
chr2:85620290-85650855:+
Coord C1 exon
chr2:85620290-85620528
Coord A exon
chr2:85639747-85639872
Coord C2 exon
chr2:85650721-85650855
Length
126 bp
Sequences
Splice sites
3' ss Seq
TATACTCCTCACTTCTTCAGATA
3' ss Score
8.91
5' ss Seq
CAGGTTTGT
5' ss Score
7.44
Exon sequences
Seq C1 exon
ATGACCCTGAGGCACGAAGATGGCGATGAGACCGCTGACCCCCCTCCCAGTGGGCGCAAGGACAGAAGAAGGGCCAGTCTGGGTGGCAGTGAGCGAAGGGGTCTGGAGCGCCGACGGAGCCGCAGGCACTCAGTACAGAACGTCAAAAGCACTTTATCAGCTCCCGCCAGTCCATGCAGTCAGTCAAACCCCAGCTTCATGCTAGGCCACGGACAGCGCGTGGATGATCTCAGTGAGAG
Seq A exon
ATACAATAGCTATGGCAGCAACTCTTACTCTTCTCCGCGACCTTACTCTGGTTTGAGTGCTCCTACAACACCCACTTCTCGTTATGGGACAACTCTCTCACCACCTCAGGAGTCCAAGTCTGACAG
Seq C2 exon
GCCTGCTTTGGGTGGTCTTCTTACATCATCTTTCCGGCAGCACCAGGAGTCCTTGGCAGCAGAAAGAGAAAGACGGCGCCAAGAGAGAGAAGAAAGGTTGCAGAGGATAGAACGTGAAGAAAGAAACAAATTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000013124-'11-20,'11-19,16-20
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.977 A=1.000 C2=0.993
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGGACAGAAGAAGGGCCAGT
R:
CTTTCTGCTGCCAAGGACTCC
Band lengths:
248-374
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]