MmuEX0019239 @ mm9
Exon Skipping
Gene
ENSMUSG00000034295 | Fhod3
Description
formin homology 2 domain containing 3 [Source:MGI Symbol;Acc:MGI:1925847]
Coordinates
chr18:25160292-25214895:+
Coord C1 exon
chr18:25160292-25160533
Coord A exon
chr18:25186566-25186670
Coord C2 exon
chr18:25214761-25214895
Length
105 bp
Sequences
Splice sites
3' ss Seq
CCAACCTCTGTGCCTTTTAGATA
3' ss Score
8.7
5' ss Seq
CAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GTGGCACTCAGGCATGAAGATGGTGATGAGACAGCTGAGCCACCCCCCAGTGGGCACCGGGACCGGAGGAGGGCCAGCATGTGTTCTGGTGGCACTGTGGGTGAGCAACAGGGCCTTGACCGCAGAAGGAGCCGTAGACACTCAATCCAGAATATCAAGAGCCCACTGTCAGCTCCCACCAGCCCCTGCTCCCCGTCTGTTCCTGCCTTCAAACCCAGCCAAGTTCGAGACCTCTGTGAAAA
Seq A exon
ATACAGCAATTTCAGCAGCAACTCTTTCCAATCCTCCAGACCCTCTCCTGGACCTAGTGGGTCCCCATCTTATGCATCATCCTTCTCATCCCCACAGGATACCAG
Seq C2 exon
GTCATCGCCAAGTGGCCTTCTCACATCCTCGTTCAGGCAGCACCAAGAGTCACTGGCAGCAGAGAGAGAGCGGAGGCGGCAAGAGAGAGAGGAAAGACTGCAGAGGATAGAACGGGAAGAAAGGAACAAATTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034295_MULTIEX1-3/3=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.920 A=0.958 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGATGAGACAGCTGAGCCAC
R:
GGATGTGAGAAGGCCACTTGG
Band lengths:
248-353
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: